Growth and Aspartate Kinase Activity in Wheat Cell Suspension Cultue : Effects of Lysine Analogs and Aspartate-Derived Amino Acids
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概要
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The effects of lysine analogs and aspartate-derived amino acids on the growth of wheat cell suspension culture were studied. S-(2-Aminoethyl)-L-cysteine (AEC), δ-hydroxylysine (DHL) and trans-lysene caused complete growth inhibition at 1.0 mM. The growth inhibition of lysine analogs were, in the order of decreasing effectiveness; AEC≥DHL, trans-lysene>oxalysine, homolysine and lysyne. cis-Lysene and methyl-lysine were not inhibitory even at concentrations of 10 mM. Lysine effectively relieved growth inhibition induced by the lysine analogs. Lysine plus threonine showed concerted inhibition, which was relieved by the addition of methionine. Activity of aspartate kinase extracted from wheat cell suspension culture was strongly inhibited by L-lysine; 0.75 to 1 mM of lysine was required for half-maximal inhibition. Threonine and methionine, individually or in combination with lysine, showed no inhibitory effect on the enzyme activity. S-Adenosylmethionine, when added with lysine in equimolar concentrations, enhanced the feedback inhibition by lysine, lowering the concentration of lysine for half-maximal inhibition to 0.13 mM. The aspartate kinase isolated from the cells cultured in the presence of 5 mM lysine did not differ in regulatory properties from the enzyme from the cells cultured without lysine. AEC at 5 mM inhibited the enzyme activity by 50%. Other lysine analogs were not inhibitory to the enzyme activity even at 10 mM. Growth inhibition of wheat suspension culture by aspartate-derived amino acids and lysine analogs were discussed in relation to their inhibitory effects on aspartate kinase activity.
- 日本植物生理学会の論文
著者
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Suzuki A
Department Of Agricultural Chemistry The University Of Tokyo
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Suzuki Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Hiramoto Toshirou
Device Development Center Hitachi Ltd.
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Yamada Yasuyuki
Research Centerfor Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Suzuki Akinori
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Yamada Yasuyuki
Grad. School Of Biol. Sci. Naist
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Yamada Yasuyuki
Graduate School Of Biological Science Nara Institute Of Science And Technoiogy
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Suzuki A
National Institute Of Agrobiological Resources
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Kumpaisal Rosarin
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Hashimoto Takashi
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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Sugimoto Yukihiro
Department of Agricultural Chemistry,. Faculty of Agriculture, University of Tokyo
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Yamada Y
Present Address: Graduate School Of Biological Science Nara Institute Of Science And Technology
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Sugimoto Y
Kyoto Univ. Kyoto Jpn
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Yamada Yasuyuki
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Kumpaisal Rosarin
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Hashimoto Takashi
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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HASHIMOTO Takashi
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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