Alteration of the Self-incompatibility Phenotype in Brassica by Transformation of the Antisense SLG Gene
スポンサーリンク
概要
- 論文の詳細を見る
Self-incompatible (SI) Brassica rapa (syn. B. campestris) was transformed with an antisense SLG gene by using SLG_8 cDNA isolated from the B. campestris S_8 homozygote. Two transformed lines were obtained and analyzed. Northern blot and Western blot analyses revealed that endogenous SLG and SRK were greatly reduced of the transcriptional and translational levels in the transformant. Pollination experiments confirmed that their SI phenotype had broken down. In addition, the progeny with the antisense SLG gene, resulting from self-or cross-pollination of the transgenic plant, also showed the self-compatible phenotype. The breakdown of SI in the tranformants was due to the change in property of the stigma and not of the pollen. These results provide strong evidence that SLG and/or SRK is implicated in the pollen-stigma recognition of SI and that they act only as stigmatic factors.
- 社団法人日本農芸化学会の論文
- 2000-05-23
著者
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Suzuki A
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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ISOGAI Akira
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Suzuki A
Department Of Agricultural Chemistry The Uniersity Of Tokyo
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柴 博史
奈良先端科学技術大学院大学バイオサイエンス研究科
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Suzuki Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Shiba Hiroshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Takayama Seiji
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Saika Hiroaki
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Takayama S
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Takayama Seiji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Suzuki Akinori
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Suzuki Atsushi
Biodevelopment Division Central Institute Nagoya Seiraku Co. Ltd.
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HINATA Kokichi
Research Institute of Seed Production Co. Ltd
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Isogai A
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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KIMURA Nobuko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Isogai Akira
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Shiozawa H
Department Of Agricultural Chemistry The University Of Tokyo
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Hinata K
Iwate Biotechnology Research Center
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Shiozawa H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Kimura Nobuko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Shiba Hiroshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Suzuki Akinori
Chemistry Faculty Of Agriculture The University Of Tokyo
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Isogai Akira
Department Of Applied Biological Chemistry The University Of Tokyo
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Isogai Akira
Graduate School Of Agricultural And Life Science The University Of Tokyo
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Suzuki A
Faculty Of Agriculture The University Of Tokyo
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