S-Locus-Specific Glycoproteins Associated with Self-Incompatibility in Brassica campestris

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概要

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• Specific S-glycoproteins were isolated from three Brassica campestris cultivars homozygous with respect to the S-alleles S_8, S_9 and S_<12>. Amino acid sequences of various peptide fragments of the S-proteins were determined using a gas-phase protein sequencer, and a nearly complete amino acid sequence of the S_8-glycoprotein was determined on the basis of the revised cDNA sequence of the B. oleracea S-specific glycoprotein. The lysyl endopeptidase fragments of S_9 and S_<12>-glycoproteins were aligned in comparison with the sequence of the S_8-glycoprotein. Although extensive sequence homology was evident among the three S-glycoproteins, the sequences of the middle part were relatively different from each other. The numbers and positions of N-glycosylation also differed among the S-glycoproteins of Brassica species.

著者

• Suzuki A

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Suzuki Akinori

  Department Of Agricultural Chemistry The University Of Tokyo

• Ueda Yukari

  Department Of Agricultural Chemistry The University Of Tokyo

• Saika Hiroaki

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• ISOGAI Akira

  Department of Agricutural Chemistry, The University of Tokyo

• Takayama S

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Takayama Seiji

  Department Of Internal Medicine Funabashi Central Hospital

• HINATA Kokichi

  Faculty of Agriculture, Tohoku University

• Suzuki Akinori

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Ueda Yoshinori

  Department Of Agricultural Chemistry The University Of Tokyo

• TSUKAMOTO Chise

  Department of Agricultural Chemistry, The University of Tokyo

• SHIOZAWA Hideyuki

  Department of Agricultural Chemistry, The University of Tokyo

• OKAZAKI Keiichi

  Faculty of Agriculture, Tohoku University

• Shiozawa H

  Department Of Agricultural Chemistry The University Of Tokyo

• Okazaki K

  Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University

• Isogai Akira

  Department Of Agricultural Chemistry The University Of Tokyo

• Hinata Kokichi

  Faculty Of Agriculture Tohoku University

• Tsukamoto C

  Department Of Agricultural Chemistry The University Of Tokyo

• Tsukamoto Chise

  Department Of Agricultural Chemistry The University Of Tokyo

• Shiozawa H

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Tsukamoto C

  Laboratory Of Food Biotechnology Faculty Of Agriculture Tohoku University

• Isogai Akira

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Okazaki Keiichi

  Faculty of Agriculture, Niigata University

• TAKAYAMA Seiji

  Department of Agricultural Chemistry, The University of Tokyo

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