Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray

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概要

• 論文の詳細を見る

• The complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. To understand these molecular mechanisms, we performed cDNA microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. Microarray analysis of 4,304 cDNA clones revealed that the hybridization signal of 396 cDNA clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of leaves. Cluster analysis with the expression data showed that 259 cDNA clones (156 non redundant groups) were specifically or predominantly expressed in anther tissues and were regulated by developmental stage-specific manners in the anther tissues. These co-regulated genes would be impor-tant for development of functional anther tissues. Furthermore, we selected several clones for RNA in situ hybridization analysis. From these analyses, we found several novel genes that show temporal and spatial expression patterns during anther development in addition to anther-specific genes reported so far. These results indicate that the genes identified in this experiment are controlled by different programs and are specialized in their developmental and cell types.

• 日本遺伝学会の論文
• 2004-08-25

著者

• Watanabe M

  Lab. Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku Univ.

• Takahashi Hideyuki

  Graduate School Of Life Sciences Tohoku University

• 渡辺 正勝

  岡崎国立共同研究機構基礎生物学研究所

• FUKUDA Hiroo

  Department of Biological Sciences, Graduate School of Science, The University of Tokyo

• DEMURA Taku

  Plant Science Center, RIKEN

• Mizuho Hidetoshi

  Faculty Of Agriculture Iwate University

• Endo Makoto

  Lab. Of Biotechnology National Inst. Of Crop Sci.

• Demura Taku

  Plant Sci. Centre

• Fukuda Hiroo

  Department Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Saika Hiroaki

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• HIGASHITANI Atsushi

  Graduate School of Life Sciences, Tohoku University

• MIZUNO Hidetoshi

  Graduate School of Life Sciences, Tohoku University

• Endo Makoto

  Faculty Of Agriculture Miyazaki University

• SAITO Hiroshi

  Faculty of Pharmaceutical Sciences, Tokyo University

• MASUKO Hiromi

  Faculty of Agriculture, Iwate University

• HAKOZAKI Hirokazu

  Faculty of Agriculture, Iwate University

• KAMADA Motoshi

  Graduate School of Life Sciences, Tohoku University

• WATANABE Masao

  Faculty of Agriculture, Iwate University

• TSUCHIYA Tohru

  Life Science Research Center, Mie University

• MATSUBARA Hitoshi

  Faculty of Agriculture, Iwate University

• HAKOZAKI Hirokazu

  Graduate School of Life Sciences, Tohoku University

• Higashitani A

  Kagoshima University Graduate School Of Medical And Dental Sciences Space Environmental Medicine

• Shiozawa H

  Department Of Agricultural Chemistry The University Of Tokyo

• Kamada Motoshi

  Graduate School Of Life Sciences Tohoku University

• Masuko Hiromi

  Faculty Of Agriculture Iwate University

• Tsuchiya T

  Life Science Research Center Mie University

• Tsuchiya Tohru

  Life Science Research Center Mie University

• Kamada Motoshi

  Graduate School Of Agriculture And Biological Science Osaka Prefecture University

• Higashitani Atsushi

  Graduate School Of Life Sciences Tohoku Univ.

• Shiozawa H

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Takahashi Hideyuki

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Masuko Hiromi

  Graduate School Of Life Sciences Tohoku University

• Endo M

  Lab. Of Biotechnology National Inst. Of Crop Sci.

• Tsuji Hiroyuki

  National Agricultural Research Center For Hokkaido Region (narch)

• Fukuda Hiroo

  Deparment Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Watanabe Masao

  Faculty Of Agriculture Iwate University

• Higashitani Atsushi

  Kagoshima University Graduate School Of Medical And Dental Sciences Space Environmental Medicine

• Hakozaki Hirokazu

  Graduate School Of Life Sciences Tohoku University

• Takahashi Hirokazu

  Laboratory Of Plant Molecular Genetics Graduate School Of Agricultural And Life Sciences The Univers

• Takahashi Hirokazu

  School Of Agriculture Meiji University

• Takahashi Hirokazu

  Laboratory Of Plant Molecular Genetics Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Saito Hiroshi

  Faculty Of Agriculture Iwate University

• ENDO Makoto

  Faculty of Liberal Arts, Dokkyo University

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