Molecular Cloning of Members of the S-Multigene Family in Self-Incompatible Brassica campestris L. : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION

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We isolated 12 groups of genomic clones that contained SLG-homologous regions from a genomic library constructed from an S^9 homozygote of self-incompatible Brassica campestris. Both SLG^9 and SRK^9 genomic clones, which are located within the self-incompatibility (S) locus, were included in these groups. The promoter regions of SLG^9 and SRK^9 were completely identical for at least 200 bp upstream from their respective initiation codons (ATG). The five sequence elements (boxes I to V) that are conserved in the promoters of SLG and SRK genes were also found in the SLG^9 and SRK^9 clones. However, one conserved element (box III) unexpectedly lacked 7 of 11 bp, although box III has been considered necessary for expression in pistil. The other ten groups of genomic clones were classified into six SRK-like groups and four SLG-like groups. These results indicate that SLG. SRK. SLG-like, and SRK-like genes form a large S-multigene family in B. campestris.
日本植物生理学会の論文