Molecular Cloning of Members of the S-Multigene Family in Self-Incompatible Brassica campestris L. : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
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概要
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We isolated 12 groups of genomic clones that contained SLG-homologous regions from a genomic library constructed from an S^9 homozygote of self-incompatible Brassica campestris. Both SLG^9 and SRK^9 genomic clones, which are located within the self-incompatibility (S) locus, were included in these groups. The promoter regions of SLG^9 and SRK^9 were completely identical for at least 200 bp upstream from their respective initiation codons (ATG). The five sequence elements (boxes I to V) that are conserved in the promoters of SLG and SRK genes were also found in the SLG^9 and SRK^9 clones. However, one conserved element (box III) unexpectedly lacked 7 of 11 bp, although box III has been considered necessary for expression in pistil. The other ten groups of genomic clones were classified into six SRK-like groups and four SLG-like groups. These results indicate that SLG. SRK. SLG-like, and SRK-like genes form a large S-multigene family in B. campestris.
- 日本植物生理学会の論文
著者
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Watanabe M
Lab. Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku Univ.
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Suzuki G
Div. Of Natural Sci. Osaka Kyoiku Univ.
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Toriyama K
Tohoku Univ. Sendai Jpn
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Toriyama Kinya
Faculty Of Agriculture Tohoku University
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Suzuki G
Division Of Natural Science Osaka Kyoiku University
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WATANABE Masao
Faculty of Agriculture, Iwate University
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Suzuki Go
Faculty of Agriculture, Tohoku University
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Isogai Akira
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Hinata K
Iwate Biotechnology Research Center
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Hinata Kokichi
Faculty Of Agriculture Tohoku University
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Isogai Akira
Department Of Applied Biological Chemistry The University Of Tokyo
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Isogai Akira
Graduate School Of Agricultural And Life Science The University Of Tokyo
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Watanabe Masao
Faculty Of Agriculture Iwate University
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