Homology-dependent suppression of stigma phenotype by an antisense S-locus glycoprotein (SLG) gene in Brassica rapa L.

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概要

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• Self-incompatibility(SI)in Brassicaceae is sporophytically controlled by haplotypes of the polymorphic S locus complex. Two tightly linked polymorphic genes at the S locus, S-locus glycoprotein(SLG)and S-receptor kinase(SRK)genes, are specifically expressed in the stigma. S-haplotypes have been classified into class I and class II types based on the sequence similarity of their SLGs, and their SRKs. To investigate the effect of an antisense SLG gene on the class divergency of the endogenous SLG and SRK genes, we introduced an antisense class I SLG^<43> cDNA into a cultivar Osome in Brassica rapa which was heterozygous for class I S^<52> haplotype and class II S^<60>. SLG^<43> is more similar to the endogenous SLG^<52>(87.8% identity)than to SLG^<60>(74.8% identity). Out of ten primary transformants analyzed, two were completely self-compatible ; the SI phenotype of stigma was altered from S^<52>S^<60> to S^<60> but that of pollen was not. In these two plants, the expression levels of mRNA and protein of SLG^<52> were reduced, whereas those of SLG^<60> were not. We suggest that an antisense class I SLG-transgene causes homology-dependent suppression, which leads to breakdown of the class I S-haplotype specificity in stigma but not the class II S-haplotype.

• 日本育種学会の論文

著者

• Watanabe M

  Lab. Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku Univ.

• Toriyama K

  Tohoku Univ. Sendai Jpn

• Toriyama Kinya

  Graduate School Of Agric. Sci. Tohoku Univ.

• WATANABE Masao

  Faculty of Agriculture, Iwate University

• HATAKEYAMA Katsunori

  Research Institute of Seed Production Co. Ltd

• HINATA Kokichi

  Research Institute of Seed Production Co. Ltd

• Takasaki Takeshi

  Research Institute of Seed Production Co., Ltd.

• Takasaki Takeshi

  Faculty Of Agriculture Kobe University

• Hinata K

  Iwate Biotechnology Research Center

• Takeuchi Takuji

  Faculty Of Science Tohoku University

• Takasaki T

  Faculty Of Agriculture Kobe University

• Watanabe Masao

  Faculty Of Agriculture Iwate University

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