Molecular Characterization of a 313-kb Genomic Region Containing the Self-incompatibility Locus of Ipomoea trifida, a Diploid Relative of Sweet Potato
スポンサーリンク
概要
- 論文の詳細を見る
Diploid Ipomoea trifida is an ancestral wild species of the cultivated hexaploid sweet potato, and displays a sporophytic self-incompatibility (SI) that is controlled by a single multiallelic S-locus. To characterize the genomic region of the S-locus using a map-based cloning method, a BAC library consisting of approximately 40,000 clones was constructed from genomic DNA of S_1-homozygote, and screened using S-linked DNA markers which were mapped in our previous study. We constructed a contig covering the S-locus region with additional screening of fosmid and λ phase libraries. RFLP analysis of recombinant plants using terminal end sequences of the BAC clones as probes indicated that the S-locus region was delimited within a map distance of 0.57 cM, spanning approximately 300 kb in physical distance. Remarkable suppression of genetic recombination was detected in the S-locus region. From sequence analysis of the 313-kp region, 43 ORFs, many repetitive sequences and 5 transposable elements were predicted. None of the ORFs, however, showed a high homology with the SI genes reported to date at the S-locus of other plant families, suggesting that a unique molecular mechanism is involved in the SI system of the Convolvulaceae family.
- 日本育種学会の論文
著者
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Suzuki Go
Division of Natural Science, Osaka Kyoiku University
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Suzuki G
Div. Of Natural Sci. Osaka Kyoiku Univ.
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Suzuki Go
Division Of Natural Science Osaka Kyoiku University
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Kowyama Yasuo
Faculty ofBioresources, Mie Uleiversity
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Suzuki G
Division Of Natural Science Osaka Kyoiku University
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TSUCHIYA Tohru
Life Science Research Center, Mie University
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Kowyama Yasuo
Faculty Of Bioresources Mie University
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Kakeda Katsuyuki
Faculty of Bioresources, Mie University
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Mukai Y
Division Of Natural Science Osaka Kyoiku University
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Tomita Rubens
Faculty Of Bioresources Mie University
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Kowyama Y
Faculty Of Bioresources Mie University
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Mukai Yasuhiko
Division Of Natural Sciences Department Of Arts And Sciences Osaka Kyoiku University
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Mukai Yasuhiko
Osaka Kyoiku University
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Tsuchiya Tohru
Life Science Research Center Mie University
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Yoshida Kazuo
Faculty of Bioresources, Mie University
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Yano Yukihito
Faculty of Bioresources, Mie University
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Yano Yukihito
Faculty Of Bioresources Mie University
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Kakeda K
Faculty Of Bioresources Mie University
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Kakeda Katsuyuki
Faculty Of Bioresources Mie University
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Yoshida Kazuo
Faculty Of Bioresources Mie University
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Kowyama Yasuo
Faculty Of Bioresource Mie University
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Mukai Yasuhiko
Division Of Natural Science Osaka Kyoiku University
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