A Pistil-Specific PR-1 Like Protein of Camellia, Its Expression, Sequence and Genealogical Position

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A major protein endogenously expressed in Camellia pistils with a high level of expression at the period of anthesis was detected by two-dimensional gel electrophoresis. Their N-terminal amino acid sequences showed a high level of honrology with pathogenesis-related (PR)-1 proteins from plants. Northern blot analysis demonstrated that the protein was expressed specifically in pistils. The gene coding the PR-1-Iike protein was cloned from tea (Camellia sinensis), and compared with those of other PR-1 and PR-1-Iike proteins. Based on sequence homology and induction, the protein was designated as Camellia PR-1-Iike (PRL-1) protein. The deduced PRL-1 protein sequence displayed the highest homology with STS14, which was earlier described as a PR-1-Iike protein expressed in pistils of potato. With the addition of this PRL-1 protein, we discussed the possibility of a new class of PR-1-Iike proteins, which are specifically induced in pistils of many plaut families. The sequence of PRL-1 gene is registered as Accession: AB015047.
日本育種学会の論文