Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana

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概要

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• The 3-ketoacyl-ACP synthase (KAS) II is a fatty-acid-related enzyme which catalyzes the elongation of 16:0-acyl carrier protein (ACP) to 18:0-ACP in plastids. The fatty acid biosynthesis 1-1 (fab1-1) mutant of Arabidopsis thaliana is partially deficient in its activity of Arabidopsis thaliana 3-ketoacyl-ACP synthase 2 (AtKAS2), and its phenotype has been intensively studied in connection with the chilling resistance and fatty acid composition. In this study, we used the T-DNA insertion mutant of AtKAS2 to examine its possible role in plant development. Reverse transcription (RT)-PCR showed that the AtKAS2 gene was expressed in various plant organs, except for roots, and was highly expressed in siliques. The fusion of β-glucuronidase (GUS) to the AtKAS2 promoter demonstrated that the promoter was active in various tissues such as embryos, stomatal guard cells, inflorescences and pollen grains. We were not able to identify atkas2 homozygous mutant adult plants in heterozygous mutant progeny. Phenotypic and genetic analyses showed that disruption of the AtKAS2 by T-DNA insertion caused embryo lethality, and the development of the embryos was arrested at the globular stage. Taken together, our results suggest that AtKAS2 is required for embryo development in Arabidopsis during the transition from the globular to the heart stage.

• 日本遺伝学会の論文
• 2008-04-25

著者

• Watanabe M

  Lab. Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku Univ.

• KAZAMA Tomohiko

  Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit

• 渡辺 正勝

  岡崎国立共同研究機構基礎生物学研究所

• Takada Yoshinobu

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Suzuki Go

  Division of Natural Science, Osaka Kyoiku University

• Watanabe Masao

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Park J‐i

  Graduate School Of Life Sciences Tohoku Univ.

• Suzuki G

  Div. Of Natural Sci. Osaka Kyoiku Univ.

• Suzuki Go

  Division Of Natural Science Osaka Kyoiku University

• Endo Makoto

  Lab. Of Biotechnology National Inst. Of Crop Sci.

• Endo Makoto

  Laboratory Of Fish Health Management Department Of Marine Biosciences Tokyo University Of Marine Sci

• KAWAGISHI KOBAYASHI

  Laboratory of Biotechnology, National Institute of Crop Science

• Suzuki G

  Division Of Natural Science Osaka Kyoiku University

• HAKOZAKI Hirokazu

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• PARK Jong-In

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• TAKEDA Yoshimitsu

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• HAKOZAKI Hirokazu

  Graduate School of Life Sciences, Tohoku University

• Kakizaki Tomohiro

  Laboratory Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku University

• Kazama Tomohiko

  Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University

• Takahata Yoshihito

  Laboratory Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku University

• Takada Yoshinobu

  Faculty Of Agriculture Iwate University

• Takada Yoshinobu

  Laboratory Of Plant Breeding Faculty Of Agriculture Iwate University

• Kawagishi Kobayashi

  Laboratory Of Biotechnology National Institute Of Crop Science

• Endo Makoto

  Laboratory Of Biotechnology National Institute Of Crop Science

• Kazama Tomohiko

  Graduate School Of Life Sciences Tohoku University

• Endo M

  Lab. Of Biotechnology National Inst. Of Crop Sci.

• Watanabe Masao

  Laboratory Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku University

• Hakozaki Hirokazu

  Graduate School Of Life Sciences Tohoku University

• ENDO Makoto

  Faculty of Liberal Arts, Dokkyo University

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