Cytoplasmic male sterility-associated ORF79 is toxic to plant regeneration when expressed with mitochondrial targeting sequence of ATPase γ subunit

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概要

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• Cytoplasmic male sterility (CMS) is a maternally inherited trait that results in the inability of plants to produce functional pollen. CMS is considered as a phenomenon caused by aberrant mitochondrial genomes. In BT-type CMS of rice (Oryza sativa L.), a CMS-associated mitochondrial gene has been reported to be an orf79 gene. To confirm the effect of ORF79 on cell fate, we introduced the orf79 gene with or without a mitochondrial targeting sequence into Taichung 65 carrying normal cytoplasm. It was revealed that ORF79 was toxic to plant regeneration when expressed as a fusion with mitochondrial targeting sequence of the ATPase γ subunit. This result implicates ORF79 as cytotoxic, and its toxicity depends on its combination with a mitochondrial targeting sequence.

• 2010-03-25

著者

• Toriyama Kinya

  Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit

• KOJIMA Hisayo

  Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit

• KAZAMA Tomohiko

  Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit

• FUJII Sota

  Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit

• Fujii Sota

  Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University

• Kojima Hisayo

  Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University

• Toriyama Kinya

  Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University

• Kazama Tomohiko

  Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University

関連論文

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