Analyses of the Genomic Sequence and Promoter Activity of a Gene for a Protein Similar to Tat Binding Protein Isolated from Brassica rapa
スポンサーリンク
概要
- 論文の詳細を見る
We have previously isolated a cDNA clone, which is similar in sequence to the Tat binding protein (TBP) of human immunodeficiency virus 1, from the anthers of Brassica rapa and designated it BrTBP. In the present study we isolated a genomic clone of BrTBP from B. rapa and found several sequence motifs conserved in various pollen-expressed genes. The 1.7-kb promoter region of BrTBP was fused to the GUS gene and introduced into tobacco and Arabidopsis thaliana. GUS expression was observed exclusively in mature pollen after anthesis in transgenic tobacco, while it was observed in tricellular pollen starting two days before anthesis in A. thaliana. Such expression patterns at the late stage of pollen development have not been reported in other pollen-specific promoters. These results indicate that the BrTBP promoter region used in this study has a unique activation pattern in tobacco and A. thaliana.
- 日本植物細胞分子生物学会の論文
- 2001-03-01
著者
-
Toriyama Kinya
Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University
-
Kitashiba Hiroyasu
Laboratory Of Plant Breeding And Genetics Faculty Of Agriculture Tohoku University
-
Toriyama Kinya
Laboratory Of Plant Breeding And Genetics Faculty Of Agriculture Tohoku University
-
OTA Reiko
Laboratory of Plant Breeding and Genetics, Faculty of Agriculture, Tohoku University
-
Ota Reiko
Laboratory Of Plant Breeding And Genetics Faculty Of Agriculture Tohoku University
-
Ota Rieko
Laboratory of Plant Breeding and Genetics, Faculty of Agriculture, Tohoku University
関連論文
- Cytoplasmic male sterility-associated ORF79 is toxic to plant regeneration when expressed with mitochondrial targeting sequence of ATPase γ subunit
- Gene trap strategy, an effective tool for identification of novel genes expressed in anther tissues in Arabidopsis thaliana
- Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid
- Abolition of the Tapetum Suicide Program Ruins Microsporogenesis
- An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings
- Expression of Bra r 1 Gene in Transgenic Tobacco and Bra r 1 Promoter Activity in Pollen of Various Plant Species
- Localization of the Ca^-Binding Protein, Bra r 1, in Anthers and Pollen Tubes
- Frequency and Pattern of Transposition of the Maize Transposable Element Ds in Transgenic Rice Plants
- A cDNA Clone, Encoding a New NBS-LRR Protein, Expressed in Immature Anthers and Leaves of Rice Plant
- Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice
- Effects of Amino Acid Medium on Cell Aggregation in Suspension-cultured Rice Cells
- Alternation of the Accumulation of Alternative Oxidase by Introducing Sense and Antisense OsAOXla in Rice
- Enhanced high temperature tolerance in transgenic rice seedlings with elevated levels of alternative oxidase, OsAOX1a
- Accumulation of raffinose in rice seedlings overexpressing OsWRKY11 in relation to desiccation tolerance
- Ultrastructural Characterization of Exine Development of the transient defective exine 1 Mutant Suggests the Existence of a Factor Involved in Constructing Reticulate Exine Architecture from Sporopollenin Aggregates
- Cytoplasmic-Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines
- Cytoplasmic Male Sterility-Related Protein Kinase, OsNek3, is Regulated Downstream of Mitochondrial Protein Phosphatase 2C, DCW11
- Analyses of the Genomic Sequence and Promoter Activity of a Gene for a Protein Similar to Tat Binding Protein Isolated from Brassica rapa
- DCW11, Down-Regulated Gene 11 in CW-Type Cytoplasmic Male Sterile Rice, Encoding Mitochondrial Protein Phosphatase 2C is Related to Cytoplasmic Male Sterility