Localization of the Ca^<2+>-Binding Protein, Bra r 1, in Anthers and Pollen Tubes
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概要
- 論文の詳細を見る
Calcium plays an essential role during pollen development and pollen tube growth, and several Ca^<2+>-binding proteins are expressed in anthers. We have previously reported that Brassica pollen allergens encoded by Bra r 1 and Bra r 2 show sequence similarities to Ca^<2+>-binding proteins [Toriyama et al.(1995) Plant Mol.Biol.29: 1157]. Herein, we report that both genes are expressed in the diploid tapetum and haploid microspores, as detected by in situ RNA hybridization. Immunoblot analysis revealed that Bra r 1 and Bra r 2 were accumulated in anthers during pollen development. When pollen grains were suspended in an aqueous solution, both proteins were mainly detected in the pollen extracellular fraction, indicating that Bra r 1 and Bra r 2 are released from the pollen upon hydration. Localization of Bra r 1 was further investigated in sections of anthers and pollen tubes. Bra r 1 was detected in the tapetum, microspores and pollen grains. In longitudinal sections of cross-pollinated pistils, Bra r 1 was detected throughout pollen tubes elongating in transmitting-tissue. These findings suggest that Bra r 1 may be involved in pollen-pistil interaction and pollen tube growth.
- 日本植物生理学会の論文
著者
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Toriyama Kinya
Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit
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Toriyama Kinya
Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University
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Toriyama Kinya
Laboratory Of Plant Breeding And Genetics Graduate School Of Agricultural Science Tohoku University
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Okada Takashi
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku Universit
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Zhang Zhaojie
Department of Botany and Microbiology, University of Oklahoma
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Russell Scott
Department of Botany and Microbiology, University of Oklahoma
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Zhang Zhaojie
Department Of Botany And Microbiology University Of Oklahoma
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Russell Scott
Department Of Botany & Microbiology University Of Oklahoma
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Russell Scott
Department Of Botany And Microbiology University Of Oklahoma
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Okada T
Tohoku Univ. Sendai Jpn
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Okada Takashi
Laboratory Of Plant Breeding And Genetics Graduate School Of Agricultural Science Tohoku University
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Toriyama Kinya
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University
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