Frequency and Pattern of Transposition of the Maize Transposable Element Ds in Transgenic Rice Plants
スポンサーリンク
概要
- 論文の詳細を見る
Two kinds of T-DNA constructs, I-RS/dAc-I-RS and Hm^RDs, carrying a non-autonomous transposable element of Ac of maize were introduced into rice plants by Agrobacterium-mediated gene transfer. Six transgenic rice plants identified as containing a single copy of the element were crossed with two transgenic rice plants carrying a gene for Ac transposase under the control of the cauliflower mosaic virus 35S promoter. In F_2 progenies, excision of the element was detected by PCR analysis and re-integration of the element was investigated by Southern blot analysis. The frequency of the excision of the element was found to vary from 0 to 70% depending on the crossing combination. The frequency of the number of individual transposition events out of the total number of F_2 plants with germinal excision was 44% in one crossing combination and 38% in the other. In the most efficient case, 10 plants with independent transposition were obtained out of the 49 F_2 plants tested. Linkage analysis of the empty donor site and the transposed Ds-insertion site in F_3 plants demonstrated that one of five Ds-insertion sites was not linked to the empty donor site. The transgenic rice obtained in this study can be used for functional genomics of rice.
- 日本植物生理学会の論文
著者
-
Toriyama Kinya
Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku Universit
-
Toriyama Kinya
Laboratory Of Environmental Biotechnology Graduate School Of Agricultural Science Tohoku University
-
Machida Yasunori
Laboratory Of Developmental Biology Division Of Biological Science Graduate School Of Science Nagoya
-
Machida Chiyoko
Laboratory Of Developmental Biology Division Of Biological Science Graduate School Of Science Nagoya
-
Nakagawa Yuko
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku Universit
-
Nakagawa Yuko
Laboratory Of Plant Breeding And Genetics Graduate School Of Agricultural Science Tohoku University
関連論文
- Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid
- Cytoplasmic male sterility-associated ORF79 is toxic to plant regeneration when expressed with mitochondrial targeting sequence of ATPase γ subunit
- Gene trap strategy, an effective tool for identification of novel genes expressed in anther tissues in Arabidopsis thaliana
- Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid
- Abolition of the Tapetum Suicide Program Ruins Microsporogenesis
- An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings
- Expression of Bra r 1 Gene in Transgenic Tobacco and Bra r 1 Promoter Activity in Pollen of Various Plant Species
- Localization of the Ca^-Binding Protein, Bra r 1, in Anthers and Pollen Tubes
- Frequency and Pattern of Transposition of the Maize Transposable Element Ds in Transgenic Rice Plants
- A cDNA Clone, Encoding a New NBS-LRR Protein, Expressed in Immature Anthers and Leaves of Rice Plant
- Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice
- Effects of Amino Acid Medium on Cell Aggregation in Suspension-cultured Rice Cells
- Alternation of the Accumulation of Alternative Oxidase by Introducing Sense and Antisense OsAOXla in Rice
- The transposition pattern of the Ac element in tobacco cultured cells
- Enhanced high temperature tolerance in transgenic rice seedlings with elevated levels of alternative oxidase, OsAOX1a
- Accumulation of raffinose in rice seedlings overexpressing OsWRKY11 in relation to desiccation tolerance
- Ultrastructural Characterization of Exine Development of the transient defective exine 1 Mutant Suggests the Existence of a Factor Involved in Constructing Reticulate Exine Architecture from Sporopollenin Aggregates
- Cytoplasmic-Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines
- Cytoplasmic Male Sterility-Related Protein Kinase, OsNek3, is Regulated Downstream of Mitochondrial Protein Phosphatase 2C, DCW11
- Analyses of the Genomic Sequence and Promoter Activity of a Gene for a Protein Similar to Tat Binding Protein Isolated from Brassica rapa
- DCW11, Down-Regulated Gene 11 in CW-Type Cytoplasmic Male Sterile Rice, Encoding Mitochondrial Protein Phosphatase 2C is Related to Cytoplasmic Male Sterility