Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations

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概要

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• Self-incompatibility (SI) in Brassicaceae is sporophytically controlled by a single S-locus with multi allelic variety. The male S determinant, SP11/SCR (S-locus protein 11/S-locus cysteine-rich protein), is a small cysteine-rich protein, and the female S determinant, SRK (S-locus receptor kinase), functions as a receptor for SP11 at the surface of stigma papilla cells. Although a few of the following downstream factors in the SP11-SRK signaling cascade have been identified, a comprehensive understanding of the SI mechanism still remains unexplained in Brassicaceae. Analysis of self-compatible (SC) mutants is significant for understanding the molecular mechanism in SI reactions, thus we screened SC lines from a variety of Japanese bulk-populations of B. rapa vegetables. Two lines, TSC4 and TSC28, seem to have disruptions in the SI signaling cascade, while the other line, TSC2, seems to have a deficiency in a female S determinant, SRK. In TSC4 and TSC28, known SI-related factors, i.e. SRK, SP11, MLPK (M-locus protein kinase), THL (thioredoxin-h-like), and ARC1 (arm repeat containing 1), were expressed normally, and their expression levels were comparable with those in SI lines. On a B. rapa genetic linkage map, potential SC genes in TSC4 and TSC28 were mapped on linkage groups A3 and A1, respectively, whereas MLPK, ARC1, and THL were mapped on A3, A4, and A6, respectively. Although potential SC genes of TSC4 and MLPK were on the same linkage group, their positions were apparently independent. These results indicate that the SC genes of TSC4 and TSC28 are independent from the S-locus or known SI-related genes. Thus, the SC lines selected here have mutations in novel factors of the SI signaling cascade, and they will contribute to fill pieces in a signal transduction pathway of the SI system in Brassicaceae.

著者

• ISOGAI Akira

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Isokawa Sachiyo

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Osaka Masaaki

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Shirasawa Akira

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Kikuta Rina

  Faculty of Agriculture, Iwate University

• Komatsu Satoshi

  Faculty of Agriculture, Iwate University

• Horisaki Atsushi

  Tohoku Seed Co. Ltd.

• Niikura Satoshi

  Tohoku Seed Co. Ltd.

• Takada Yoshinobu

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Shiba Hiroshi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Takayama Seiji

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Suzuki Go

  Division of Natural Science, Osaka Kyoiku University

• Suwabe Keita

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Watanabe Masao

  Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University

• Suzuki Go

  Division Of Natural Science Osaka Kyoiku University

• Suwabe Keita

  Laboratory Of Plant Reproductive Genetics Graduate School Of Life Sciences Tohoku University

• Takayama Seiji

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Niikura Satoshi

  Tohoku Seed Co.

• Takada Yoshinobu

  Laboratory Of Plant Breeding Faculty Of Agriculture Iwate University

• Shiba Hiroshi

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Isogai Akira

  Graduate School Of Agricultural And Life Science The University Of Tokyo

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