Resistance to Protoporphyrinogen Oxidase-inhibiting Compound S23142 from Overproduction of Mitochondrial Protoporphyrinogen Oxidase by Gene Amplification in Photomixotrophic Tobacco Cells(Organic Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Tobacco VZI-1S cells exhibit a 150-fold greater resistance to the protoporphyrinogen oxidase (Protox)-inhibiting compound, S23142, from wild-type tobacco cells. To investigate the mechanism for this S23142 resistance, the protein level, enzymatic activity, and sensitivity to S23142 in two Protox isoenzymes (plastidal and mitochondrial forms) were examined. The level of mitochondrial Protox protein was greater, and its activity 5-times higher, in YZI-IS cells than in wild-type cells. Furthermore, the apparent IC_<50> value of S23142 was about 20 nM, which is 20-fold higher than that observed in wild-type cells. In contrast, no differences were found in the plastidal Protox protein level, activity or its inhibition by S23142 between YZI-1S and wild-type cells. A southern blot analysis revealed that the mitochondrial Protox gene had been significantly amplified in the YZI-1S cells. These results suggest that the S23142 resistance of YZI-1S cells was due to the overproduction of mitochondrial Protox by gene amplification.
- 社団法人日本農芸化学会の論文
- 2002-09-23
著者
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Yoshida Shigeo
Plant Science Center, RIKEN
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Watanabe N
Faculty Of Agriculture Shizuoka University
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ISOGAI Akira
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Takayama Seiji
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Che Fang-sik
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Yoshida S
Plant Science Center Riken Institute
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Yoshida S
Cancer Immunotherapy Center Nagoya Kyoritu Hospital
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Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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Takayama S
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Takayama Seiji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Suzuki Y
Department Of Applied Biological Chemistry The University Of Tokyo
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Che Fang-sik
Graduate School Of Biological Sciences Nara Inst. For Sci. And Technol.
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WATANABE Naohide
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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Yoshida Shigeo
Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)
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Suzuki Yoshihito
Department Of Applied Biological Chemistry The University Of Tokyo
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Isogai A
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Suzuki Y
Grad. Sch. Bioagric. Sci. Nagoya Univ.
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Che Fang-sik
Department Of Agricultural Chemistry The University Of Tokyo
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Isogai Akira
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Che Fang-sik
Graduate School Of Bio-science Nagahama Institute Of Bio-science And Technology
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Isogai Akira
Department Of Applied Biological Chemistry The University Of Tokyo
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Isogai Akira
Graduate School Of Agricultural And Life Science The University Of Tokyo
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Yoshida Shigeo
Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)
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Yoshida Shigeo
Plant Funct. Lab.
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