Purification from Conditioned Medium and Chemical Identification of a Factor That Inhibits Somatic Embryogenesis in Carrot

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概要

• 論文の詳細を見る

• Somatic embryogenesis is strongly inhibited in cultures of carrot(Daucus carota L.)cells when the cell density is high. The inhibition is caused by factors that are released by cells into the medium of such cultures. In this study, we purified and identified one of the inhibitory factors found in the medium of high-cell-density cultures of carrot cells. The inhibitory factor with the strongest apparent activity was purified by fractionation with ethylacetate, chromatography on an octadecylsilyl(ODS) silica gel-column and HPLC. The inhibitory factor had a single peak of absorbance at 280 nm and was identified as 4-hydroxybenzyl alcohol by mass spectrometry and ^1H-and ^<13>C-NMR spectroscopy. Authentic 4-hydroxybenzyl alcohol strongly inhibited the formation of somatic embryos at a concentration equal to that in high-cell-density cultures. These results suggest that 4-hydroxybenzyl alcohol is a major factor that accumulates in high-cell-density cultures of carrot cells and inhibits somatic embryogenesis.

• 日本植物生理学会の論文

著者

• Yoshida Shigeo

  Plant Science Center, RIKEN

• 吉田 静夫

  北大低温研

• 吉田 静夫

  Discovery Res. Inst. Riken

• 吉田 静夫

  北海道大学低温科学研究所

• 吉田 静夫

  北大・低温研

• ASAMI Tadao

  Riken

• Asami T

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Asami T

  The Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Asami Tadao

  Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)

• Yoshida S

  Riken Kanagawa Jpn

• Kamada H

  Univ. Tsukuba Tsukuba Jpn

• YOSHIDA Shigeo

  Institute of Physical and Chemical Research

• KAMADA Hiroshi

  Institute of Biological Sciences, University of Tsukuba

• Sasaki Kazuo

  Department Of Neuropsychiatry Fukui Medical University

• Kobayashi Toshihiro

  Institute of Biological Sciences, University of Tsukuba

• Higashi K

  Gene Research Center University Of Tsukuba

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Higashi Katsumi

  Institute Of Biological Sciences University Of Tsukuba

• Asami Tadao

  Plant Funct. Lab.

• Sasaki K

  Hokkaido Univ. Sapporo Jpn

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Sasaki K

  Department Of Bioscience And Biotechnology Faculty Of Engineering Aomori University

• Kamada Hiroshi

  Institute Of Biological Sciences Univ.

• Kobayashi T

  Institute Of Biological Sciences University Of Tsukuba

• Asami Tadao

  Riken Saitama Jpn

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Yoshida Shigeo

  Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)

• Yoshida Shigeo

  Plant Funct. Lab.

• Sasaki Kazuo

  Department Of Applied Physics Tohoku University

• Kamada Hiroshi

  Institute Of Biological Sciences Tsukuba University

• Sasaki Kazuo

  Department Of Applied Chemistry Faculty Of Engineering Hiroshima University

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