Establishment of a new Zinnia experimental system including separation of the distinct cell population with flow cytometry and its culture
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概要
- 論文の詳細を見る
An in vitro culture system of Zinnia mesophyll cells has best used for studies on xylogenesis. Our previous studies with Zinnia cell culture demonstrated the presence of different cell types in the culture and their cell-cell communication. As the first step to investigate such cell-cell communication, we intended to establish a method to separate different cell populations and to culture a specific one. Zinnia mesophyll cells were stained with fluorescein diacetate, and then the fluorescence-positive and negative-cells were separated successfully using flow cytometric cell sorting. The fluorescence-positive cells differentiated into tracheary elements in the presence of an antibiotic, nalidixic acid. These procedures will be useful not only for the analysis of cell-cell interactions in Zinnia culture, but also for separating cells with intact cell walls in other plant species.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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Yoshida Shigeo
Plant Science Center, RIKEN
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福田 裕穂
東大 大学院理学系研究科
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福田 裕穂
東京大学大学院理学系研究科
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FUKUDA Hiroo
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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福田 裕穂
東大 理 植物園
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Ito Yasuko
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Fukuda Hiroo
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Fukuda Hiroo
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Fukuda H
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Yoshida Shigeo
Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)
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Fukuda Hiroo
Riken Plant Science Center
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INO Reiko
Biomedical Research, Beckman Coulter K. K.
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Ino Reiko
Biomedical Research Beckman Coulter K. K.
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Yoshida Shigeo
Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)
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Fukuda Hiroo
Deparment Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Yoshida Shigeo
Plant Funct. Lab.
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Yoshida Shigeo
Plant Functions Laboratory, RIKEN institute
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