Chlorophyll-deficient Mutants of Rice Demonstrated the Deletion of a DNA Fragment by Heavy-ion Irradiation

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概要

• 論文の詳細を見る

• Heavy-ion irradiation is a new method of mutation breeding to produce new cultivars. We established the application of this method in rice plants to obtain mutants. Rice seeds were irradiated by C or Ne ions (135MeV/u) with a LET (linear energy transfer) of 22.7 or 64.2 keV/μm, respectively. Chlorophyll-deficient mutants (CDM) segregated in M_2 progeny were albino, pale-green, yellow or striped-leave phenotypes. The highest rate of CDM with C-ion irradiation, 7.31%, was obtained at 40 Gy among the doses examined. Ne-ion irradiation gave the highest rate, 11.6%, at 20 Gy. We used the RLGS (Restriction Landmark Genomic Scanning) method to analyze DNA deletion in an albino mutant genome. Not I-landmark RLGS profiles detected about 2000 spots in rice. We found that one of the polymorphic spots was strongly linked to the albino phenotypic mutant derived from deleting of a DNA fragment, and demonstrated the high ability to detect of polymorphic regions by the RLGS method.

• 日本放射線影響学会の論文

著者

• Yoshida Shigeo

  Plant Science Center, RIKEN

• 吉田 静夫

  北大低温研

• 吉田 静夫

  Discovery Res. Inst. Riken

• 吉田 静夫

  北海道大学低温科学研究所

• 吉田 静夫

  北大・低温研

• Yoshida S

  Riken Kanagawa Jpn

• Suzuki Yoshikatsu

  Plant Science Center Riken Institute

• Yamaguchi I

  Department Of Applied Biological Chemistry The University Of Tokyo

• KAMEYA Toshiaki

  Graduate School of Life Sciences, Tohoku University

• Sekido S

  Plant Science Center Riken

• Sekido Shigeko

  Microbial Toxicology Laboratory And Biodesign Research Group Riken(the Institute Of Physical And Che

• ABE TOMOKO

  Plant Functions Lab. RIKEN

• MATSUYAMA TOMOKI

  Plant Functions Lab. RIKEN

• SEKIDO SHIGEKO

  Plant Science Center, RIKEN

• YAMAGUCHI ISAMU

  Plant Science Center, RIKEN

• ABE Tomoko

  Institute of Physical and Chemical Research

• YOSHIDA Shigeo

  Institute of Physical and Chemical Research

• Kameya T

  Graduate School Of Life Sciences Tohoku University

• Suzuki Yoshihito

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Yamaguchi Isamu

  Laboratory For Remediation Research Plant Science Center Riken

• Abe T

  Plant Science Center Riken.

• Yamaguchi Isamu

  Plant Science Center Riken

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Kameya Toshiaki

  Graduate School Of Life Sciences Tohoku Univ.

• Yoshida S

  Plant Science Center Riken.

• Shimada Yukihisa

  Plant Science Center Riken

• Yoshida Saiko

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Abe Tomoko

  Plant Function Laboratory Institute Of Physical And Chemical Research

• Yoshida Shigeo

  Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Physical And Chemical Res.

• Yoshida Shigeo

  Plant Funct. Lab.

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