Cytokinin-induced Gene Expression in Cultured Green Cells of Nicotiana tabacum Identified by Fluorescent Differential Display
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概要
- 論文の詳細を見る
The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP/GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones.
- 社団法人日本農芸化学会の論文
- 2001-06-23
著者
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Yoshida Shigeo
Plant Science Center, RIKEN
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Asami Tadao
Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)
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TAKI Noriyuki
Plant Functions Lab., RIKEN Inst.
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KIMURA Takuma
Plant Functions Lab., RIKEN Inst.
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NAKANO Takeshi
Plant Functions Lab., RIKEN Inst.
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Asami Tadao
Plant Funct. Lab.
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Asami Tadao
Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)
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Yoshida Shigeo
Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)
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Kimura Takuma
Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)
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Nakano Takeshi
Plant Chemical Biology Research Unit Riken Advanced Science Institute
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ISHIKAWA Mayuko
Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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Ishikawa Mayuko
Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)
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Nakano Takeshi
Plant Chemical Biology Research Unit Riken
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Yoshida Shigeo
Plant Function Laboratory The Institute Of Physical And Chemical Research (riken)
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Nakano Takeshi
Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)
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Yoshida Shigeo
Plant Funct. Lab.
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Yoshida Shigeo
Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)
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Kimura Takuma
Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)
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