Identification and Semi-Quantification of Glbberellms from the Pollen and Anthers of Zea mays by Immunoassay and GC/MS
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概要
- 論文の詳細を見る
Radioimmunoassays and enzyme-linked immunosorbent assays for methyl esters of gibberellins A_1, A_3, A_4, and A_7 were established using an antiserum specific for GA_1-Me. The antiserum was characterized by high titer and specificity for such C_<19>-GAs with 3β-hydroxyl group as GA_1, GA_3, GA_4 and GA_7. Combination of this antiserum and HPLC enabled us to identify and quantify GA_1 and GA_4 from the pollen of Zea mays with a high degree of reliability. Similarly, identification and quantification of GA_9 and GA_<20> were also made possible by use of an antiserum specific for GA_<20>-Me. Combined use of immunoassays and GC/MS enabled us to identify nine GAs from the pollen and four from the anthers of Zea mays. The identification of non-13-hydroxylated GAs, such as GA_4 and GA_9, in addition to 13-hydroxylated GAs from the pollen and the anthers suggests that the early-non-hydroxylation pathway, as well as the early-13-hydroxylation pathway, operates in the male reproductive organs of Zea mays, and that the organ-specific biosynthesis and/or localization of GAs in Zea mays is similar to that in Oryza sativa.
- 日本植物生理学会の論文
著者
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TAKAHASHI Nobutaka
Department of Biochemistry University of Shizuoka School of Pharmaceutical Science
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SUZUKI Yoshihito
Department of Applied Biological Chemistry, The University of Tokyo
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YAMAGUCHI Isomaro
Department of Applied Biological Chemistry, The University of Tokyo
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MUROFUSHI Noboru
Department of Biotechnology, Akita Prefectural University
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NAKAGAWA Ryusuke
Department of Cell Regulation, Faculty of Medicine, Kagawa University
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Maejima Noboru
Department Of Biotechnology Akita Prefectural University
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Takahashi Nobutaka
Department Of Agricultural Chemistry The University Of Takyo
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Nakazawa Hideyuki
Department of Hematology, Shinshu University School of Medicine
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Suzuki Y
Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry The University Of Tokyo
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Yoshida Shigeo
Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)
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Murofushi Noboru
Department Of Agricultural Chemistry The University Of Tokyo
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Suzuki Yoshihito
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamaguchi Isomaro
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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Nakazawa Hideyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Yamaguchi I
Department Of Applied Biological Chemistry University Of Tokyo
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Suzuki Y
Research Institute For Bioresources Okayama University
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Niwa Rimpei
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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Tsuji Naoki
Department Of Clinical Laboratory Medicine Sapporo Medical University School Of Medicine
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Weiler E
Ruhr‐univ. Bochum Deu
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Kurogochi Shin
Nihon Tokushu-Noyaku Co. Ltd.
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Weiler Elmar
Department of Plant Physiology, Ruhr-University Bochum
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Nakagawa Ryusuke
Department Of Cell Regulation Faculty Of Medicine Kagawa University
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Suzuki Yoshihito
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Takahashi Nobutaka
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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