Monoclonal Antibodies Specific for Non-Derivatized Gibberellins I. Preparation of Monoclonal Antibodies against GA_4 and Their Use in Immunoaffinity Column Chromatography

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概要

• 論文の詳細を見る

• Cell lines producing monoclonal antibodies specific for non-derivatized gibberellins (GAs) were prepared from spleen cells of a mouse immunized with an immunogen that carried 16-substituted GA_4 as a hapten. The conditions for association and dissociation of antibody-hapten complexes were examined to determine the most effective procedure for immunoaffinity chromatography. The final procedure adopted was proven to be very effective by its application to the analysis of GAS in the anthers of rice and immature seeds of Phaseolus vulgaris.

• 日本植物生理学会の論文

著者

• TAKAHASHI Nobutaka

  Department of Biochemistry University of Shizuoka School of Pharmaceutical Science

• Furuya Masaki

  Frontier Research Programs Riken Institute

• Furuya Masaki

  Frontier Research Programs Riken

• Nagatani Akira

  Frontier Research Programs Riken

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• NAKAJIMA Masatoshi

  Department of Applied Biological Sciences, The University of Tokyo

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Nonaka Masahiko

  Food Research & Development Laboratories Ajinomoto Co. Inc.

• Takahashi Nobutaka

  Department Of Agricultural Chemistry The University Of Takyo

• Kizawa Satoru

  Department of Agricultural Chemistry, The University of Tokyo

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Kaihara Sumiko

  Lab.Expt.Biol., kyoyama-Hatsunecho

• Kaihara Sumiko

  Lab.expt.biol. Kyoyama-hatsunecho

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Kaihara S

  Lab.expt.biol. Kyoyama-hatsunecho

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry University Of Tokyo

• 長谷 あきら

  Department Of Botany Graduate School Of Science Kyoto University

• Tsuji Naoki

  Department Of Clinical Laboratory Medicine Sapporo Medical University School Of Medicine

• Nonaka M

  Kyushu Univ. Fukuoka Jpn

• Kizawa Satoru

  Department Of Agricultural Chemistry The University Of Tokyo

• Takahashi N

  Graduate School Of Agriculture Kyoto Univ.

• Nagatani Akira

  Frontier Research Program. Riken Inst.

• Kaihara Sumiko

  Department Of Agricultural Chemistry The University Of Tokyo

• Nonaka M

  Department Of Applied Biological Chemistry The University Of Tokyo

• Takahashi Nobutaka

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

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