Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+
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概要
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Five full-length cDNA encoding gibberellin 2-oxidases, VaGA2oxA1, VaGA2oxA2, VaGA2oxB1, VaGA2oxB2, and VaGA2oxB3, were cloned from etiolated adzuki bean (Vigna angularis cv. Dainagon) seedlings, and their enzymatic characteristics were examined using recombinant enzymes fused with glutathione S-transferase (GST). Recombinant VaGA2oxA1 (rVaGA2oxA1) and rVaGA2oxA2 showed 2β-hydroxylation activity by converting GA1, GA4, GA9, GA20, GA4-methyl ester, and 16,17-dihydro-GA4 to the corresponding 2β-hydroxylated gibberellins, which were identified by GC/MS. rVaGA2oxB1, rVaGA2oxB2, and rVaGA2oxB3 showed similar activity by converting [3H4]-16,17-dihydro-GA4 to a metabolite showing an Rf value of 16,17-dihydro-GA34. RNA-blot analysis showed that VaGA2oxA1 and VaGA2oxA2 were the major ones expressed in etiolated hypocotyls. The addition of Co2+ instead of Fe2+ to the assay medium apparently reduced the enzymatic activity, but increased the binding of [3H4]-16,17-dihydro-GA4 to rVaGA2oxA1, indicating the possibility that VaGA2oxs can be detected as gibberellin-binding proteins under certain conditions.
- 社団法人 日本農芸化学会の論文
著者
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YAMAGUCHI Isomaro
Department of Applied Biological Chemistry, The University of Tokyo
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Park Seung-hyun
Department Of Applied Biological Chemistry The University Of Tokyo
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NAKAJIMA Masatoshi
Department of Applied Biological Sciences, The University of Tokyo
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Sakane Masayuki
Department of Applied Biological Chemistry, University of Tokyo
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XU Zheng-Jun
Rice Institute, Sichuan Agricultural University
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TOMIOKA Katsushi
Department of Applied Biological Chemistry, University of Tokyo
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Yamaguchi Isomaro
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry University Of Tokyo
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Nakayama M
Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.
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Xu Zheng-jun
Rice Institute Sichuan Agricultural University
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Nonaka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Xu Zheng-jun
Rice Inst. Sichuan Agricultural Univ.
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