Immunohistochemistry of Gibberellins in Rice Anthers

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概要

• 論文の詳細を見る

• A new immunohistochemical method for analyzing the localization of gibberellins was developed and applied to rice anthers. This method uses 1, 3-diisopropylcarbodiimide gas fixation to prevent the drift of gibberellins during the fixation procedure. Spikelets before anthesis were frozen in liquid nitrogen and lyophilized. They were then fixed with diisopropylcarbodiimide gas in a desiccator, which catalyzed amide formation between the 7-carboxyl groups of gibberellins and the amino groups of proteins in the tissue. The fixed spikelets were embedded in paraffin and sectioned, the sections being stained with the anti-GA 1-Me antibody and visualized by using an immunoperoxidase system. The anthers just before anthesis were stained well, while those 4-5days before heading were not stained. We analyzed the gibberellins in an extract of the rice anthers by an immunoassay, using the anti-GA 1-Me antibody. On the basis of the results, we conclude that the major substances stained in this immunohistochemical study should be 16α, 17-dihydroxy-16, 17-dihydrogibberellin A4-17-O-β-D-glucoside and 16α, 17-dihydroxy-16, 17-dihydrogibberellin A7-17-O-β- D-glucoside.

• 社団法人日本農芸化学会の論文
• 1995-10-23

著者

• ZHANG Jun

  Department of Pharmacology, Tokyo Medical University

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• NAKAJIMA Masatoshi

  Department of Applied Biological Sciences, The University of Tokyo

• HASEGAWA Morifumi

  Department of Bioresource Science, College of Agriculture, Ibaraki University

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• TAKEDA Kazuyoshi

  Research Institute for Bioresources, Okayama University

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Hashimoto Nobuyoshi

  Department of Applied Biological Chemistry, The University of Tokyo

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Hashimoto Nobuyoshi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Zhang Jun

  Department Of Chemistry Korea University

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Nakayama M

  Juntendo Univ. School Of Medicine Tokyo Jpn

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry University Of Tokyo

• Takeda K

  Faculty Of Pharmaceutical Sciences Tokyo University Of Science

• Zhang J

  Tohoku Univ. Sendai‐shi Jpn

• Nonaka M

  Department Of Applied Biological Chemistry The University Of Tokyo

• Takeda Kazuyoshi

  Research Institute For Bioresources Okayama University

• Zhang Jun

  Department Of Bioengineering And Robotics Graduate School Of Engineering Tohoku University

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• Zhang Jun

  Department Of Anesthesiology Huashan Hospital Fudan University

• Takeda Kazuyoshi

  Research Institute Bioresource Okayama University

• Hasegawa Morifumi

  Laboratory of Agricultural Chemicals, Faculty of Agriculture, Ibaraki University

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