New Assay Method for Protoporphyrinogen Oxidase Inhibitors Using Chloroplasts Isolated from Spinacia oleracea L.
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概要
- 論文の詳細を見る
A new assay method for protoporphyrinogen oxidase inhibitors, using stromal extracts of chloroplasts and a fluorescence micro-plate reader, was developed. The I_<50> value of S-23142 which had been confirmed to a specific protoporphyrinogen oxidase inhibitor was 10^<-10> M (100 pM) in this assay. These results indicate that this new assay system is very useful and sensitive for screening protoporphyrinogen oxidase inhibtors.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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ASAMI Tadao
Riken
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Asami T
Dep. Of Applied Biological Chemistry Univ. Of Tokyo
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MUROFUSHI Noboru
Faculty of Bioresource Sciences, Akita Prefectural University
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ASAMI Tadao
The Institute of Physical and Chemical Research (RIKEN)
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YOSHIDA Shigeo
The Institute of Physical and Chemical Research (RIKEN)
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WANG Jing-Ming
Department of Biotechnology, Akita Prefectural University
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MUROFUSHI Noboru
Department of Biotechnology, Akita Prefectural University
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Che Fang-sik
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Wang Jing-ming
Department Of Biotechnology Akita Prefectural University
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Maejima Noboru
Department Of Biotechnology Akita Prefectural University
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Che Fang-sik
Graduate School Of Biological Sciences Nara Inst. For Sci. And Technol.
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Murofushi Noboru
Department Of Agricultural Chemistry The University Of Tokyo
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Che Fang-Sik
The Institute of Physical and Chemical Research (RIKEN)
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Che Fang-sik
Department Of Agricultural Chemistry The University Of Tokyo
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Asami Tadao
Riken Saitama Jpn
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