Qualitative and Quantitative Analysis of Endogenous Gibberellins in Onion Plants and Their Effects on Bulb Development

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概要

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• Evidence has been reported that bulb development in onion plants (Allium cepa L.) is controlled by endogenous bulbing and anti-bulbing hormones, and that gibberellin (GA) is a candidate for anti-bulbing hormone (ABH). In this study, we identified a series of C-13-H GAs (GA_<12>, GA_<15>, GA_<24>, GA_9, GA_4, GA_<34>, and 3-epi-GA_4) and a series of C-13-OH GAs (GA_<44>, GA_<20>, GA_1, and GA_8) from the leaf sheaths including the lower part of leaf blades of onion plants (cv. Senshu-Chuko). These results suggested that two independent GA biosynthetic pathways, the early-non-hydroxylation pathway to GA_4 (active GA) and early-13-hydroxylation pathway to GA_1 (active GA), exist in onion plants. It was also suggested that GA_4 and GA_1 have almost the same ability to inhibit bulb development in onion plants induced by treatment with an inhibitor of GA biosynthesis, uniconazole-P. The endogenous levels of GA_1 and GA_4, and their direct precursors, GA_<20> and GA_9, in leaf blades, leaf sheaths, and roots of 4-week-old bulbing and non-bulbing onion plants were measured by gas chromatography/selected ion monitoring with the corresponding [^2H]labeled GAs as internal standards. In most cases, the GA levels in long-day (LD)-grown bulbing onion plants were higher than those of short-day (SD)-grown non-bulbing onion plants, but the GA_1 level in leaf blades of SD-grown onion plants was rather higher than that of LD-grown onion plants. Relationship between the endogenous GAs and bulb development in onion plants is discussed.

• 社団法人日本農芸化学会の論文
• 1993-12-23

著者

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• SETO Hideharu

  The Institute of Physical and Chemical Research (RIKEN)

• Shibaoka H

  Osaka Univ. Toyonaka Jpn

• Shibaoka Hiroh

  Dept. Biol. Grad. Sch. Sci. Osaka Univ.

• Shibaoka Hiroh

  Department Of Biology Faculty Of Science Osaka University

• Nojiri Hideaki

  Department Of Applied Biological Chemistry The University Of Tokyo

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Noguchi Haruko

  Biotechnology Research Center The University Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Toyomasu Tomonobu

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bi

• Toyomasu T

  Department Of Bioresource Engineering Yamagata University

• Seto H

  Riken (the Institute Of Physical And Chemical Research)

• Yada H

  Biotechnology Research Center University Of Tokyo

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

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