ATP-Sensitive Binding to Microtubules of Polypeptides Extracted from Isolated Phragmoplasts of Tobacco BY-2 Cells : STRUCTURE AND FUNCTION OF CELLS
スポンサーリンク
概要
- 論文の詳細を見る
Polypeptides of about 100 kDa, with the ability to bind to microtubules assembled in vitro, were extracted from phragmoplasts isolated from tobacco cultured cells by treatment with ATP. These 100-kDa polypeptides could also be extracted from isolated spindles but not from isolated interphase nuclei, an indication of the association in vivo of these polypeptides with the micro-tubules of the spindle and of the phragmoplast. The association of the polypeptides with micro-tubules was sensitive both to ATP and to AMPPNP, a nonhydrolyzable analogue of ATP, but not to GTP. Since the 100-kDa polypeptides showed little or no ATPase activity, it seems unlikely that they are species of microtubule-dependent motility proteins.
- 日本植物生理学会の論文
著者
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Shibaoka Hiroh
Department of Biology, GraduateSchool of Science, Osaka University
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Shibaoka H
Osaka Univ. Toyonaka Jpn
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Shibaoka Hiroh
Dept. Biol. Grad. Sch. Sci. Osaka Univ.
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Shibaoka Hiroh
Department Of Biology Faculty Of Science Osaka University
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Sonobe S
Graduate School Of Life Science University Of Hyogo
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Sonobe Seiji
Department Of Biology Faculty Of Science Osaka University
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YASUHARA Hiroki
Department of Biotechnology Faculty of Engineering, Kansai University
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Yasuhara H
Kansai Univ. Osaka Jpn
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Yasuhara Hiroki
Department Of Biotechnology Faculty Of Engineering And High Technology Research Center (hrc) Kansai
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Yasuhara Hiroki
Department of Biology, Faculty of Science, Osaka University
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