ATP-Sensitive Binding to Microtubules of Polypeptides Extracted from Isolated Phragmoplasts of Tobacco BY-2 Cells : STRUCTURE AND FUNCTION OF CELLS

元データ 日本植物生理学会


Polypeptides of about 100 kDa, with the ability to bind to microtubules assembled in vitro, were extracted from phragmoplasts isolated from tobacco cultured cells by treatment with ATP. These 100-kDa polypeptides could also be extracted from isolated spindles but not from isolated interphase nuclei, an indication of the association in vivo of these polypeptides with the micro-tubules of the spindle and of the phragmoplast. The association of the polypeptides with micro-tubules was sensitive both to ATP and to AMPPNP, a nonhydrolyzable analogue of ATP, but not to GTP. Since the 100-kDa polypeptides showed little or no ATPase activity, it seems unlikely that they are species of microtubule-dependent motility proteins.


Shibaoka Hiroh Department of Biology, GraduateSchool of Science, Osaka University
Shibaoka H Osaka Univ. Toyonaka Jpn
Shibaoka Hiroh Dept. Biol. Grad. Sch. Sci. Osaka Univ.
Shibaoka Hiroh Department Of Biology Faculty Of Science Osaka University
Sonobe S Graduate School Of Life Science University Of Hyogo
Sonobe Seiji Department Of Biology Faculty Of Science Osaka University
YASUHARA Hiroki Department of Biotechnology Faculty of Engineering, Kansai University
Yasuhara H Kansai Univ. Osaka Jpn
Yasuhara Hiroki Department Of Biotechnology Faculty Of Engineering And High Technology Research Center (hrc) Kansai
Yasuhara Hiroki Department of Biology, Faculty of Science, Osaka University