The Roles of the Cytoskeleton and the Cell Wall in Nuclear Positioning in Tobacco BY-2 Cells
スポンサーリンク
概要
- 論文の詳細を見る
During the course of cell-wall regeneration in protoplasts isolated from tobacco BY-2 cells, the nucleus changed its position from the central region to the cell periphery. This nuclear migration was inhibited by 2,6-dichlorobenzonitrile (DBN), suggesting the involvement of cell walls in nuclear migration in tobacco BY-2 cells. In spherical cells formed by culturing protoplasts in the presence of DBN or propyzamide, the nucleus was located in the central region of the cells and was tethered by transvacuolar cytoplasmic strands. Nuclei in the spherical cells were displaced by disrupting the actin filaments in the cytoplasmic strands by treating the cells with cytochalasin B (CB), suggesting that the position of the nucleus in the spherical cells is maintained by actin filaments. As the nuclei were located in the central region of the cells even in the presence of propyzamide, microtubules seem not to be involved in nuclear positioning in the spherical cells. Actin filaments, but not microtubules, also seem to play an important role in nuclear positioning in elongated cells. In these cells, CB greatly enhanced the displacement of the nucleus by centrifugation, while propyzamide showed little effect.
- 日本植物生理学会の論文
著者
-
Shibaoka Hiroh
Department Of Biology Faculty Of Science Osaka University
-
Katsuta J
Osaka Univ. Toyonaka Jpn
-
Katsuta Junko
Department Of Biology Faculty Of Science Osaka University
関連論文
- Gibberellin A_3 Causes a Decrease in the Accumulation of mRNA for ACC Oxidase and in the Activity of the Enzyme in Azuki Bean (Vigna angularis) Epicotyls
- Actinomycin D Inhibits the GA_3-Induced Elongation of Azuki Bean Epicotyls and the Reorientation of Cortical Microtubules
- Synthesis of Polysaccharides in Phragmoplasts Isolated from Tobacco BY-2 Cells
- A New Method for Preservation of Actin Filaments in Higher Plant Cells
- Calcium-Sensitivity of Cortical Microtubules in the Green Alga Mougeotia
- Effects of Gibberellin on the Arrangement and the Cold Stability of Cortical Microtubules in Epidermal Cells of Pea Internodes
- Brassinosteroids Act as Regulators of Tracheary-Element Differentiation in Isolated Zinnia Mesophyll Cells
- Assembly of Microtubules in a Cytoplasmic Extract of Tobacco BY-2 Miniprotoplasts in the Absence of Microtubule-Stabilizing Agents
- Inhibition of Cell Division and DNA Synthesis by Gibberellin in Isolated Zinnia Mesophyll Cells
- Stabilization of Cortical Microtubules in Maize Mesocotyl Cells by Gibberellin A_3 : GROWTH AND DEVELTOPMENT : STRUCTURE AND FUNCTION OF CELLS
- Isolation of Casparian Strips from Pea Roots : STRUCTURE AND FUNCTION OF CELLS
- Nuclear-Cycle Dependence of the Development of the Preprophase Band in Tobacco BY-2 Cells
- Effects of Root Excision on Swelling of Leaf Sheath Cells and on the Arrangement of Cortical Microtubules in Onion Seedlings
- Effects of S-3307, an Inhibitor of Gibberellin Biosynthesis, on Swelling of Leaf Sheath Cells and on the Arrangement of Cortical Microtubules in Onion Seedlings
- Changes in Microtubules in Onion Leaf Sheath Cells during Bulb Development
- Plasma Membrane Ghosts Form Differently When Produced from Microtubule-Free Tobacco BY-2 Cells
- Effects of Taxol on the Development of the Cell Plate and of the Phragmoplast in Tobacco BY-2 Cells
- ATP-Sensitive Binding to Microtubules of Polypeptides Extracted from Isolated Phragmoplasts of Tobacco BY-2 Cells : STRUCTURE AND FUNCTION OF CELLS
- Inhibition of Cell-Plate Formation by Brefeldin A Inhibited the Depolymerization of Microtubules in the Central Region of the Phragmoplast
- Effects of Propyzamide on Tobacco Cell Microtubules In Vivo and In Vitro
- Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. 2,6-Dichlorobenzonitrile as a new cellulose-synthesis inhibitor
- Qualitative and Quantitative Analysis of Endogenous Gibberellins in Onion Plants and Their Effects on Bulb Development
- Qualitative and Quantitative Analysis of Endogenous Jasmonic Acid in Bulbing and Non-Bulbing Onion Plants : GROWTH AND DEVELOPMENT
- Effects of Actinomycin D and 2,4-Dinitrophenol on the Development of Root Primordia in Azuki Bean Stem Cuttings
- Growth and cell wall changes in azuki bean epicotyls II.Changes in wall polysaccharides during auxin-induced growth of excised segments
- The Roles of the Cytoskeleton and the Cell Wall in Nuclear Positioning in Tobacco BY-2 Cells
- Involvement of wall microtubules in gibberellin promotion and kinetin inhibition of stem elongation
- Gibberellin-colchicine interaction in elongation of azuki bean epicotyl sections
- Increases in the number of adventitious roots caused by 2-thiouracil and 5-bromodeoxyuridine in Phaseolus mungo cuttings
- The nature of the dual effect of auxin on root formation in Azukia cuttings
- Anatomical and physiological aspects of developmental processes of adventitious root formation in Azukia cuttings
- Morphological and physiological characterization of IAA-less-sensitive and IAA-sensitive phases in rooting of Azukia cuttings
- Portulal : a rooting promoting substance in Portulaca leaves
- Gibberellin A_3 and Abscisic Acid Cause the Reorientation of Cortical Microtubules in Epicotyl Cells of the Decapitated Dwarf Pea
- A Possible Double Role for Brassinolide in the Reorientation of Cortical Microtubules in the Epidermal Cells of Azuki Bean Epicotyls : GROWTH AND DEVELOPMENT : STRUCTURE AND FUNCTION OF CELLS
- Effects of indoleacetic, p-chlorophenoxyisobutyric and 2,4,6-trichlorophenoxyacetic acids on three phases of rooting in Azukia cuttings