Effects of Propyzamide on Tobacco Cell Microtubules In Vivo and In Vitro
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概要
- 論文の詳細を見る
Treatment with propyzamide at 2 × 10^<-6> M or at higher concentrations arrested the cell cycle at metaphase in tobacco BY-2 cells. Metaphase cells having disorganized spindle microtubules and scattered chromosomes began to appear within several minutes of the addition of propyzamide. Within 30 min, disrupted spindle microtubules and dispersed chromosomes were seen in all metaphase cells. Propyzamide at 2 × 10^<-6> M Or at higher concentrations also disrupted cortical microtubules, but disruption of cortical microtubules required more time than disruption of spindle microtubules. The effect of propyzamide on microtubules was found to be readily reversible. The cells arrested at metaphase by 2 × 10^<-6> M propyzamide resumed mitosis within 2 h from the termination of treatment with propyzamide. Spindle microtubules reappeared within 15 min from the termination of treatment with propyzamide, and the cortical microtubules within 1 h. Tubulin was isolated from tobacco BY-2 cells by column chromatography on ethyl N-phenylcarbamate-Sepharose 4B. On incubation with EGTA, Mg^<2+> and DMSO, the purified tobacco tubulin polymerized into microtubules. Propyzamide at 1 × 10^<-4> M completely inhibited the polymerization of tobacco tubulin, but did not inhibit polymerization of bovine brain tubulin. Tobacco tubulin was adsorbed onto a column of propyzamide-analogue-linked Sepharose 4B and then purified by chromatography on this column.
- 日本植物生理学会の論文
著者
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Shibaoka Hiroh
Department of Biology, GraduateSchool of Science, Osaka University
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Mizuno K
Nagoya Univ. Nagoya Jpn
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Shibaoka Hiroh
Department Of Biology Faculty Of Science Osaka University
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Shibaoka H
Department Of Biology Faculty Of Science Osaka University
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MIZUNO KOICHI
Department of Orthopedic and Spinal Surgery, Graduate School of Tokyo Medical and Dental University
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Izumi Kazuo
Pesticides Research Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.
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Mizuno Koichi
Department Of Biological Sciences Graduate School Of Science Osaka University
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Adashi Tomohiro
Department of Biology, Faculty of Science, Osaka University
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Nagano Eiki
Pesticides Research Laboratory, Takarazuka Research Center, Sumitomo Chemical Co. Ltd.
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Enomoto Masayuki
Pesticides Research Laboratory, Takarazuka Research Center, Sumitomo Chemical Co. Ltd.
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Nagano Eiki
Pesticides Research Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.
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Enomoto Masayuki
Pesticides Research Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.
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Adashi Tomohiro
Department Of Biology Faculty Of Science Osaka University
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Mizuno Koichi
Department Of Biol. Graduate School Of Sci. Osaka Univ.
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