Plasma Membrane Ghosts Form Differently When Produced from Microtubule-Free Tobacco BY-2 Cells

概要

論文の詳細を見る
When lysed in an actin stabilizing buffer, protoplasts made from tobacco BY-2 suspension culture cells formed plasma membrane ghosts that retained both corticalo actin and microtubules. Distinct cytoskeletal arrays occurred:the most common ghost array (type I) derived from protoplasts in interphase and had random actin and microtubules, although the alignment of the actin was dependent, at least partially, on microtubule organization. Type II ghosts were larger and more irregular in shape than type I ghosts, and were characterized by a lack of microtubules and the presence of distinctive arrays of actin bundles in concentric arcs. These ghosts derived from protoplasts lacking cortical microtubules produced when wall digestion occurred while the cells were in cell division, or from protoplasts isolated in the presence of 100 μM propyzamide. Because type II ghosts derived from protoplasts of similar size to those that give rise to type I ghosts, and because type II ghosts retained ordered actin arrays while the parent protoplasts had random cortical actin, type II ghosts apparently from differently to type I ghosts. We speculate that instead of the protoplast being sheared off to produce a round ghost, the plasma membrane tears and collapses onto the slide, ordering the actin bundles in the process. One implication of this model would be that cortical microtubules provide structural supprt to the plasma membrane of the protoplast so that only in their absence do the type II ghosts form.
日本植物生理学会の論文

Plasma Membrane Ghosts Form Differently When Produced from Microtubule-Free Tobacco BY-2 Cells

スポンサーリンク

概要

著者

関連論文

スポンサーリンク