Stabilization of Cortical Microtubules in Maize Mesocotyl Cells by Gibberellin A_3 : GROWTH AND DEVELTOPMENT : STRUCTURE AND FUNCTION OF CELLS

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Effects of GA_3 on the stability of cortical microtubules (MTs) were studied in mesocotyl cells of etiolated maize seedlings. Propyzamide, an MT-disrupting agent specific for plant tubulin, disrupted cortical MTs in cells in the upper regions of mesocotyls of GA_3-untreated seedlings and caused swelling of the cells. GA_3 prevented propyzamide from disrupting MTs and from causing such swelling. Chilling of mesocotyls at 4℃ for 60 min brought about the disruption of cortical MTs in cells in the upper regions of mesocotyls of GA_3-untreated seedlings, but not in cells in corresponding regions of GA_3-treated seedlings, suggesting that treatment with GA_3 increased the stability of cortical MTs in maize mesocotyl cells. Cortical MTs in protoplasts isolated from mesocotyls of GA_3-untreated seedlings failed to withstand chilling at 0℃ for 90 min, while those in protoplasts isolated from mesocotyls of GA_3-treated seedlings withstood chilling successfully. It appears that the cell wall is not involved in the stabilization of cortical MTs by GA_3 in maize mesocotyl cells.
日本植物生理学会の論文