Mechanism of Inhibition of Cytoplasmic Streaming by Auxin in Root Hair Cells of Hydrocharis

元データ 日本植物生理学会

概要

It has been reported that auxin accelerates cytoplasmic streaming at low concentrations and inhibits it at high concentrations in several plant cells. In the present study, the mechanism of inhibition of cytoplasmic streaming by naphthalene acetic acid(NAA) at high concentrations was analyzed in root hair cells of Hydrocharis. Because the effective concentration of NAA inhibiting cytoplasmic streaming decreased when the extracellular pH(pHo) was lowered, it was hypothesized that cytoplasmic streaming is inhibited by NAA via acidification of the cytoplasm. This possibility was supported by the fact that acetic acid, propionic acid and decanoic acid also inhibited cytoplasmic streaming at low pHo. Acidification of the cytoplasm disturbed the orientation of actin filaments(AFs) and disrupted cortical microtubules(MTs). The effects of NAA were reversible; both cytoplasmic streaming and organization of the cytoskeleton were recovered upon removal of NAA. During the recovery, tracks of cytoplasmic streaming in the subcortical region temporarily showed a helical pattern along the longitudinal direction of the cell. Fluorescence staining of cytoskelectons revealed that both AFs and MTs aligned obliquely to the longitudinal axis of the cell. The helical streaming returned to the original reverse fountain streaming after several hours. The simultaneous changes in the organization of both cytoskeletons supported our previous report that the organization of AFs is regulated by MTs.

著者

新免 輝男 Department Of Life Science Faculty Of Science Himeji Institute Of Technology
Shimmen Teruo Department of Life Science, Faculty of Science, Himeji Institute of Technology
SONOBE Seiji Graduate School of Life Science, University of Hyogo
Sonobe S Graduate School Of Life Science University Of Hyogo
Sonobe Seiji Department Of Biology Faculty Of Science Osaka University
TOMINAGA Motoki Department of Life Science, Graduate School of Science, Himeji Institute of Technology, Harima Scien
Tominaga Motoki Kansai Advanced Research Center Communications Research Laboratory
Tominaga Motoki Department Of Life Science Faculty Of Science Himeji Institute Of Technology Harima Science Park Cit
Shimmen Teruo Department Of Biology Faculty Of Science University Of Tokyo
Shimmen Teruo Department Of Biology Faculty Of Science Osaka University:(present)department Of Botany Faculty Of S

関連論文

▼もっと見る