Inhibitory Factor(s) of Somatic Embryogenesis Regulated Suspensor Differentiation in Suspension Culture of Japanese Larch (Larix leptolepis GORDON)
スポンサーリンク
概要
- 論文の詳細を見る
Using somatic embryogenesis system of Japanese larch that develops embryos composed of embryo-proper and suspensor, the effects of high-cell-density culture and conditioned medium on the somatic embryogenesis were investigated. High-cell-density culture strongly inhibited the somatic embryogenesis. Furthermore, the conditioned medium derived from high-cell-density culture also strongly inhibited the somatic embryogenesis, especially differentiation of the suspensor. The inhibitory effect of the conditioned medium was not attributable to the depletion of nutrients, but to the accumulation of inhibitory factor(s) in the medium. The addition of activated charcoal to high-cell-density culture resulted in the formation of numerous somatic embryos with longer suspensors than in the untreated one. This treatment also resulted in the formation of numerous vacuolated cells-like suspensor on the surface surrounding the embryo-proper. These results indicate that some inhibitory factor(s) that regulates suspensor differentiation are released into the medium of high cell density.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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Kamada H
Univ. Tsukuba Tsukuba Jpn
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SETO Hideharu
The Institute of Physical and Chemical Research (RIKEN)
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UMEHARA Mikihisa
Department of Biotechnology, Fukuoka Agricultural Research Center
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OGITA Shinjiro
Enzyme Chemistry Department, Biotechnology Research Center, Toyama Prefectural University
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SASAMOTO Hamako
Graduate School of Environmental and Information Sciences, Yokohama National University
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KAMADA Hiroshi
Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba
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Ogita S
Toyama Prefectural Univ. Toyama Jpn
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Ogita Shinjiro
Enzyme Chemistry Department Biotechnology Research Center Toyama Prefectural University
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Ogita Shinjiro
Faculty Of Agriculture. Tokyo University Of Agriculture And Technology
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Shibaoka H
Osaka Univ. Toyonaka Jpn
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Higashi K
Gene Research Center University Of Tsukuba
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Higashi K
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
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Kamada H
Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Kamada H
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kamada Hiroshi
Gene Experiment Center University Of Tsukuba
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UMEHARA Mikihisa
Gene Research Center, Institute of Biological Sciences, University of Tsukuba
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OGITA Shinjiro
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
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Sasamoto Hamako
Graduate School Of Environment And Information Sciences Yokohama National University
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Kawaide Hiroshi
Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology
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Seto H
Riken (the Institute Of Physical And Chemical Research)
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Ogita Shinjiro
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
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Umehara Mikihisa
Gene Research Center Institute Of Biological Sciences University Of Tsukuba
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Kamada Hiroshi
Gene Exp. Center Univ. Tsukuba
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