Analysis of cis-Regulatory Elements in Carrot Embryo-Specific and ABA-Responsive Gene, DcECP31

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概要

• 論文の詳細を見る

• Expression of carrot embryogenic cell specific protein genes (DcECPs) that are late-embryogenesis abundant proteins is ABA-inducible and embryo-specific. The expression of some DcECPs is controlled by an embryo-specific transcription factor C-ABI3, a carrot homologue of Arabidopsis ABI3. In order to understand the molecular mechanisms regulating ABA-inducible and embryo-specific expression of DcECP genes, we carried out a detailed analysis of DcECP31 promoter with deletion analysis, base-substitution mutagenesis and electrophoretic mobility shift assay (EMSA). We identified two important elements in the promoter of DcECP31, motif X (CACACGTGGG) and motif Y (CACACGTATC), which are sufficient for the embryo-specific and ABA-inducible promoter activity. By precise EMSA analysis, it was demonstrated that a nuclear protein which has sequence specific-binding ability binds to the flanking ACGT core motifs.

• 日本植物細胞分子生物学会の論文
• 2001-03-01

著者

• Ko Sukmin

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Kamada H

  Univ. Tsukuba Tsukuba Jpn

• KAMADA Hiroshi

  Gene Research Center, Graduate School of Life and Environmental Science, University of Tsukuba

• EUN Chang-Ho

  Gene Research Center, Institute of Biological Sciences, University of Tsukuba

• Eun Chang-ho

  Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba

• Higashi K

  Gene Research Center University Of Tsukuba

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• SATOH Shinobu

  Gene Experiment Center, Institute of Biological Sciences, University of Tsukuba

• Kamada H

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Satoh Shinobu

  Gene Experiment Center Institute Of Biological Sciences University Of Tsukuba

• Kamada Hiroshi

  Gene Experiment Center University Of Tsukuba

• Satoh Shinobu

  Univ. Tsukuba Ibaraki Jpn

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Kamada Hiroshi

  Gene Exp. Center Univ. Tsukuba

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