Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System

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概要

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• Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

• 社団法人 日本農芸化学会の論文
• 2008-12-23

著者

• NOJIRI Hideaki

  Biotechnology Research Center, The University of Tokyo

• Okada Kazunori

  Biotechnology Research Center, University of Tokyo

• Sugai Yoshinori

  United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

• Miyazaki Sho

  United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

• Nojiri H

  Biotechnology Res. Center The Univ. Of Tokyo

• Higashi K

  Gene Research Center Institute Of Biological Sciences University Of Tsukuba

• Kamada H

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Okada Kazunori

  Biotechnology Res. Center Univ. Of Tokyo

• Noguchi Haruko

  Biotechnology Research Center The University Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Nojiri Hideaki

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo

• Kawaide Hiroshi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Lin Fengqiu

  Biotechnology Research Center The University Of Tokyo

• Ko Kwang-wook

  Biotechnology Research Center The University Of Tokyo

• KATSUMATA Takumi

  Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)

• SUGAI Yoshinori

  Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)

• MIYAZAKI Sho

  Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)

• Katsumata Takumi

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology (tuat)

• Miyazaki Sho

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

• Sugai Yoshinori

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

• Kamada Hiroshi

  Gene Res. Center Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Yada H

  Biotechnology Research Center University Of Tokyo

• Kawaide Hiroshi

  Institute Of Agriculture Tokyo University Of Agriculture And Technology (tuat)

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

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