A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
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概要
- 論文の詳細を見る
Meta-cleavage product hydrolase (MCP-hydrolase) is one of the key enzymes in the microbial degradation of aromatic compounds. MCP-hydrolase produces 2-hydroxypenta-2,4-dienoate and various organic acids, according to the C6 substituent of the substrate. Comprehensive analysis of the substrate specificity of the MCP-hydrolase from Pseudomonas fluorescens IP01 (CumD) was carried out by determining the kinetic parameters for nine substrates and crystal structures complexed with eight cleavage products. CumD preferred substrates with long non-branched C6 substituents, but did not effectively hydrolyze a substrate with a phenyl group. Superimposition of the complex structures indicated that benzoate was bound in a significantly different direction than other aliphatic cleavage products. The directions of the bound organic acids appeared to be related with the kcat values of the corresponding substrates. The Ile139 and Trp143 residues on helix α4 appeared to cause steric hindrance with the aromatic ring of the substrate, which hampers base-catalyzed attack by water.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
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Fushinobu Shinya
Department of Biotechnology, The University of Tokyo
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WAKAGI Takayoshi
Department of Biotechnology, The University of Tokyo
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SHOUN Hirofumi
Department of Biotechnology, The University of Tokyo
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Shoun Hirofumi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Shoun Hirofumi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Fushinobu Shinya
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Wakagi Takayoshi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Wakagi T
Univ. Tokyo Tokyo Jpn
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JUN So-Young
Department of Biotechnology, The University of Tokyo
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HIDAKA Masafumi
Department of Biotechnology, The University of Tokyo
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NOJIRI Hideaki
Biotechnology Research Center, The University of Tokyo
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YAMANE Hisakazu
Biotechnology Research Center, The University of Tokyo
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OMORI Toshio
Department of Industrial Chemistry, Shibaura Institute of Technology
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Omori T
Coll. Of Systems Engineering And Sci. Dep. Of Bioscience And Engineering Shibaura Inst. Of Technol.
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Omori Toshio
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nojiri H
Biotechnology Res. Center The Univ. Of Tokyo
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Shoun Hirofumi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi
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Noguchi Haruko
Biotechnology Research Center The University Of Tokyo
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Yamane Hisakazu
Biotechnology Res. Center The Univ. Of Tokyo
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Yamane Hisakazu
Biotechnology Res. Center Univ. Of Tokyo
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Yamane Hisakazu
Biotech. Res. Ctr. The Univ. Of Tokyo
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Nojiri Hideaki
Biotechnology Res. Center The Univ. Of Tokyo
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Jun So-young
Department Of Biotechnology The University Of Tokyo
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Hidaka Masafumi
Department Of Biotechnology The University Of Tokyo
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Yamane Hisakazu
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Yada H
Biotechnology Research Center University Of Tokyo
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Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Omori Toshio
Shibaura Institute Of Technology
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Shoun Hirofumi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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YAMANE Hisakazu
Biotech. Res. Ctr. , The Univ. of Tokyo
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