Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• In bioaugmentation technology, survival of inoculant in the treatment system is prerequisite but remains to be a crucial hurdle. In this study, we bioaugmented the denitrification tank of a piggery wastewater treatment system with the denitrifying bacterium Pseudomonas stutzeri strain TR2 in two pilot-scale experiments, with the aim of reducing nitrous oxide (N_2O), a gas of environmental concern. In the laboratory, strain TR2 grew well and survived with high concentrations of nitrite (5-10 mM) at a wide range of temperatures (28-40℃). In the first augmentation of the pilot- scale experiment, strain TR2 inoculated into the denitrification tank with conditions (30℃, 〜0.1 mM nitrite) survived only 2-5 days. In contrast, in the second augmentation with conditions determined to be favorable for the growth of the bacterium in the laboratory (40-45℃, 2-5 mM nitrite), strain TR2 survived longer than 32 days. During the time when the presence of strain TR2 was confirmed by quantitative real-time PCR, N_2O emission was maintained at a low level even under nitrite-accumulating conditions in the denitrification and nitrification tanks, which provided indirect evidence that strain TR2 can reduce N_2O in the pilot-scale system. Our results documented the effective application of growth conditions favorable for strain TR2 determined in the laboratory to maintain growth and performance of this strain in the pilot-scale reactor system and the decrease of N_2O emission as the consequence.

• 2013-01-00

著者

• Fushinobu Shinya

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Shoun Hirofumi

  Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi

• Miyauchi Keisuke

  Faculty Of Engineering Tohoku Gakuin University

• Endo Ginro

  Faculty Of Engieering Tohoku Gakuin University

• Kim Sang-wan

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Wakagi Takayoshi

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Miyahara Morio

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Yamada Takeshi

  Faculty Of Engineering Tohoku Gakuin University

• Watanabe Akira

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Kim Sang-Wan

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo:(Present office)Department of Ecological Engineering Toyohashi University of Technology

• Ikeda-Ohtsubo Wakako

  Faculty of Engineering, Tohoku Gakuin University

• Matsuoka Masaki

  Ebara Engineering Service Co., Ltd

• Miyauchi Keisuke

  Faculty of Engineering, Tohoku Gakuin University

• Fushinobu Shinya

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Wakagi Takayoshi

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Watanabe Akira

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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