Expression in Escherichia coli of the Extrinsic 18-kDa Protein of Photosystem II of Spinach : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA encoding the precursor for the 18-kDa protein of PSII of spinach was expressed in Escherichia coli. When the cell lysate was incubated at 7℃, the precursor was degraded by pro-teases of E. coli to a polypeptide of 18 kDa (P18) that consisted of the mature protein moiety plus the last four residues of the transit peptide. P18 was able to reconstitute the water-oxidizing complex of NaCl-treated PSII membranes supplemented with the 23-kDa protein. Moreover, P18 was cleaved by the prolyl endoproteinase of spinach specifically at the Pro-12-Leu-13 bond, as was the authentic 18-kDa protein. These properties of P18 indicate that the present expression system is potentially useful for studies of the substrate specificity of the endoproteinase, as well as of the structure-function relationships of the 18-kDa protein.
- 日本植物生理学会の論文
著者
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Watanabe Akira
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Nakajima Nobuyoshi
Biotechnology Research Team National Institute For Environmental Studies
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KUWABARA Tomohiko
Institute of Biological Science, University of Tsukuba
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Takeuchi Mariko
Master's Program In Biosystem Studies University Of Tsukuba
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Kondo Noriaki
Biotechnoiogy Research Team, Regional Environment Division, National Institute for Environmental stu
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Nakajima Nobuyoshi
Biotech Inst. Fac. Bioresource Sci. Akita Pref. Univ.
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Takeuchi M
Department Of Biological Sciences Graduate School Of Science University Of Tokyo:molecular Membrane
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HONDA Shuzo
Institute of Biological Sciences, University of Tsukuba
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Watanabe Akira
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Honda Shuzo
Institute Of Biological Sciences University Of Tsukuba
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Kuwabara Tomohiko
Institute Of Biological Science University Of Tsukuba
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Kuwabara Tomohiko
Institute Of Biological Sciences University Of Tsukuba:master's Program In Biosystem Studies Un
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Kondo Noriaki
Biotechnoiogy Research Team Regional Environment Division National Institute For Environmental Studi
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Kondo Noriaki
Biotechnology Research Team National Institute For Environmental Studies
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Watanabe Akira
Department Of Plant Sciences University Of Tokyo
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Watanabe Akira
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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