Import of Modified D1 Protein and its Assembly into Photosystem II by Isolated Chloroplasts

概要

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A portion of the rbcS gene that encoded the transit peptide and 20 amino acid residues of the N-terminal region of the small subunit of ribulosebisphosphate carboxylase/oxygenase was fused to the 5' end of the psbA gene which encodes the D1 protein of PSII reaction center. The chimeric gene was expressed in vitro as a 42-kDa protein, which was imported into chloroplasts isolated from pea leaves. The imported protein was processed such that the transit peptide was lost in the stroma, the resultant protein was translocated into thylakoid membranes, and the C-terminal peptide was then removed to yield a mature protein with an N-terminal extension originated from the small subunit. The mature protein appeared to be assembled into the PSII core complex, resembling the native D1 protein in terms of protein structure and topology within the membrane. Our observations indicate that the structure of the precursor to the D1 protein includes information for the proper assembly of the protein into the PSII core complex.
日本植物生理学会の論文