Isolation of a Tobacco cDNA Encoding Sar1 GTPase and Analysis of Its Dominant Mutations in Vesicular Traffic Using a Yeast Complementation System

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概要

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• The cDNA clone of NtSAR1, a gene encoding the small GTPase Sar1p which is essential for vesicle formation from the endoplasmic reticulum (ER) membrane in yeast, has been isolated from Nicotiana tabacum BY-2 cells. NtSAR1 as well as AtSAR1 cDNA isolated from Arabidopsis thaliana [d'Enfert et al.(1992)EMBO J.11:4205] could complement the lethality of the disruption of SAR1 in yeast cells in a temperature-sensitive fashion. They also suppressed yeast sec12 and sec16 temperaturesensitive mutations as yeast SAR1 does. Using this complementation system, we analyzed the phenotypes of several mutations in plant SAR1 cDNAs in yeast cells. The expression of NtSAR1 H74L and AtSAR1 N1291 showed dominant negative effect in growth over the wild-type SAR1, which was accompanied by the arrest of ER-to-Golgi transport. Such dominant mutations will be useful to analyze the role of membrane trafficking in plant cells, if their expression can be regulated conditionally.

• 日本植物生理学会の論文

著者

• Nakano Akihiko

  Molecular Membrane Biology, RIKEN

• Nakano Akihiko

  Molecular Membrane Biology Lab Riken Advanced Science Institute:dept Biological Science Graduate Sch

• SAITO Chieko

  Inst. Physic. and Chem. Res (RIKEN), Wako

• SAITO Chieko

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

• Saito C

  Inst. Physic. And Chem. Res (riken) Wako

• Saito C

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Saito Chieko

  Department Of Biological Sciences School Of Science University Of Tokyo

• Nakano A

  Hitachi Chemical Co. Ltd. Ibaraki Jpn

• Nakano Akihiko

  Molecular Membrane Biology Laboratory Riken(the Institute Of Physical And Chemical Research)

• Nakano A

  Molecular Membrane Biology Laboratory Riken (the Institute Of Physical And Chemical Research)

• Saito C

  Ehime Univ. Matsuyama Jpn

• TAKEUCHI Masaki

  Department of Applied Chemistry, Faculty of Engineering, Kanagawa University

• Tada Masahito

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

• Yashiroda Hideki

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

• Tada Masahito

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Takeuchi M

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo:molecular Membrane

• Yashiroda Hideki

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Takeuchi Masaki

  Faculty Of Integrated Arts And Sciences Hiroshima University

• Takeuchi Masaki

  Department Of Applied Chemistry Faculty Of Engineering Kanagawa University

• Tada Masahito

  Department of Astronomy, Graduate School of Science, Tohoku University

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