Characterization of p92, Karyopherin .BETA., Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes.
スポンサーリンク
概要
- 論文の詳細を見る
A 92k protein (p92) was purified from the wheat germ agglutinin-Sepharose (WGA-Sepharose) bound fraction of a rat liver nuclear envelope salt-extract by DEAE-5PW and hydroxyapatite HPLCs. Partial amino acid sequence analysis of p92 revealed that it is karyopherin β, which was found recently in the cytosolic fraction. It was shown using antip92 antiserum that the protein is present in the nuclear envelope and cytosolic fractions, in almost the same amounts, but not in other subcellular fractions of rat liver. p92 bound to N-acetylglucosamine-bearing nucleoporins (GNPs) on WGA-Sepharose, but not directly to WGA. The amount of p92 found in the rat liver nuclear envelope fraction corresponded to about 10% of the nuclear pore complex in mass, and to as much as 140 mol of p92 per mol of nuclear pore complex. Hydrodynamic analysis of the purified p92 suggested that the molecule is present as a monomer and that it is a rod-shaped molecule. The interaction of p92 and GNPs seemed to be hydrophobic and ionic. Based on these results, the participation of nuclear envelope p92 in protein nuclear transport is discussed.
- 社団法人 日本生化学会の論文
著者
-
Omata Saburo
Department Of Biocheaistry Faculty Of Science Niigata University
-
Ichimura Tohru
Department Of Applied Chemistry National Defense Academy
-
Makifuchi Shigehito
Department Of Biochemistry Faculty Of Science Niigata University
-
Takeuchi Masaki
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
-
Horigome Tsuneyoshi
Department Of Biocheaistry Faculty Of Science Niigata University
-
Kawahire Shigeru
Department of Biochemistry, Faculty of Science
-
Ichimura Tohru
Departments of Biochemistry Faculty of Science, Niigata University
-
Odani Shoji
Biology, Faculty of Science, Niigata University
関連論文
- IMMUNOHISTOCHEMICAL STUDIES OF TSH-PRODUCING CELLS IN THE PITUITARY AND EXPRESSION OF GROWTH FACTORS IN THYROIDAL PROLIF-ERATIVE LESIONS IN RATS TREATED WITH THIOUREA AND EXCESS VITAMIN A
- Point Mutations of the c-H-ras Gene in Spontaneous Pulmonary Tumors of Transgenic Mice Carrying the Human c-H-ras Gene
- FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT
- 22A-09-3 Flow cytometric analysis (FCM) of sperms taken from rats treated with nitrobenzene (NB).
- EVALUATION OF RAT SPERM BY FLOW CYTOMETRY : SIMULTANEOUS ANALYSIS OF SPERM COUNT AND SPERM VIABILITY
- COLLABORATIVE WORK TO DETERMINE AN OPTIMAL ADMINISTRATION PERIOD AND OPTIMAL PARAMETERS FOR DETECTION OF EFFECTS ON MALE FERTILITY IN RATS : MALE REPRODUCTIVE TOXICITY STUDY OF HALOPERIDOL (<SPECIAL ISSUE>TESTICULAR TOXICITY)
- Effects of Reproduction on Spontaneous Development of Endometrial Adenocarcinomas and Mammary Tumors in Donryu Rats
- Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes
- Size Distribution of Dew Droplets and Dew Formation Effect on Deposition Fluxes
- Controlling Factors of Weak Acid and Base Concentrations in Urban Dewwater : Comparison of Dew Chemistry with Rain and Fog Chemistry
- Molecular Shape and ATP Binding Activity of Rat p50, a Putative Mammalian Homologue of RuvB DNA Helicase
- EMESIS-RELATED BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES INDUCED BY CISPLATIN IN THE FERRET
- B-20 ENTERIC 5-HT LEVELS AND KARYORRHEXIS INDUCED BY ANTINEOPLASTIC AGENTS AND COPPER SULFATE OF THE FERRET
- Methods for Imaging and Analyses of Intracellular Organelles Using Fluorescent and Luminescent Proteins
- COLLABORATIVE WORK TO EVALUATE TOXICITY ON MALE REPRODUCTIVE ORGANS BY REPEATED DOSE STUDIES IN RATS : 6)2-AND 4-WEEKS ADMINISTRATION STUDY OF HALOPERIDOL
- LARGE AMOUNT OF VITAMIN A HAS NO MAJOR EFFECTS ON THYROIDAL HORMONE SYNTHESIS IN TWO-STAGE RAT THYROID CARCINOGENESIS MODEL USING N-BIS(2-HYDROXYPROPYL)NITROSAMINE AND THIOUREA
- 21C-11-1 A study on mechanisms of the enhanced thyroidal tumorigenesis in rats by large amounts of vitamin A simultaneously given with thiourea : Effects on thyroidal hormone syntheses.
- Infected Papillary Fibroelastoma Attached to the Atrial Septum
- Isolation of a Tobacco cDNA Encoding Sar1 GTPase and Analysis of Its Dominant Mutations in Vesicular Traffic Using a Yeast Complementation System
- Nicorandil Infusion Accelerates ST-Segment Resolution in Patients with Acute Myocardial Infarction : Subanalysis in SMART Study
- Purification and Molecular Shape of a 144 kDa Protein Bearig N-Acetylglucosamine residues from Rat Liver Nuclear Envelopes
- Characterization of the Binding of Nuclear Envelope Precursor Vesicles and Chromatin, and Purification of the Vesicles
- cDNA Cloning of Nuclear Localization Signal Binding Protein NBP60, a Rat Homologue of Lamin B Receptor, and Identification of Binding Sites of Human Lamin B Receptor for Nuclear Localization Signals and Chromatin^1
- Characterization of p92, Karyopherin β, Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes
- Nucleolar Protein B23 Interacts with Japanese Encephalitis Virus Core Protein and Participates in Viral Replication
- Preparation of Hyperbranched Aromatic Poly(ethersulfone)s Possessing Sulfonic Acid Terminal Groups for Polymer Electrolyte
- Lupeol Esters from the Twig Bark of Japanese Pear (Pyrus serotina Rehd.) cv. Shinko
- EFFECT OF 4-PHENYL-1, 3-DITHIA-2-THIOXO-CYCLOPENT-4-ENE ON LIVER INJURY INDUCED BY REPEATED EXPOSURE TO GALACTOSAMINE PLUS CARBON TETRACHLORIDE IN RATS
- EFFECT OF 1,3-DITHIA-2-THIOXO-CYCLOPENT-4-ENE AND ITS DERIVATIVES ON LIVER INJURY INDUCED BY CARBON TETRACHLORIDE AND OROTIC ACID IN RATS
- Single-step Purification of Pepsin-derived Monoclonal Antibody Fragments from Crude Murine Ascitic Fluids by Ceramic Hydroxyapatite High-performance Liquid Chromatography
- Does Different D-dimer Reduce the Use of Duplex Scanning to Rule out DVT in Patients with PE?
- Evaluation of fluence and pulse-duration on purpuric threshold using an extended pulse pulsed-dye laser in the treatment of port wine stains
- Comparison of Capsular Morphology between two Different Surface Structures of Silicone Implants
- Influence of H_2O on the SiO_2 Growth by Parallel-Resonant RF Plasma Oxidation
- Enhanced Growth of Silicon Dioxide Films by Parallel-Resonant RF Plasmas
- The Effects of Substituents Introduced into 9-Aminoacridine on Frameshift Mutagenicity and DNA Binding Affinity
- Frameshift Mutagenicity and DNA Intercalation of 9-Amino-2-hydroxyacridine, a Rat Liver S9 Metabolite of 9-Aminoacridine
- Proposed Two-molecule Intercalation between Neighboring Base Pairs of DNA
- Enhancement of Mutagenic Activity of 9-Aminoacridine by Introducing a Nitro Group into the Molecule
- A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II : Cell Biology
- Molecular Cloning and Splicing Isoforms of Mouse p144, a Homologue of CA150
- Molecular Cloning of Mouse p47, a second Group Mammalian RuvB DNA Helicase-Like Protein : Homology with Those from Human and Saccharomyces cerevisiae^1
- COVALENT PROTEIN MODIFICATIONS IN THE BRAIN OF METHYLNERCURY(MeHg)-TREATED RATS.(Biochemistry)(Proceedings of the Sixty-Second Annual Meeting of the Zoological Society of Japan)
- CHANGES IN PROTEIN PHOSPHORYLATION IN THE BRAIN OF THE RAT AFTER IN VIVO METHYLMERCURY TREATMENT : Biochemistry
- Reversed-Phase High-Performance Liquid Chromatography of Membrane Proteins on Perfusion-type Polystyrene Resin Columns in 60% Formic Acid
- Effect of Butylated Hydroxytoluene on Cell Population in Rat Hepatocytes
- Characteristic Formation of Edge Transport Barrier in the Compact Helical System
- First Observation of High Density Edge Transport Barrier Formation during Reheat Mode of Helical Plasma in CHS
- Characterization of p92, Karyopherin .BETA., Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes.
- Molecular Cloning of Mouse p47, a Second Group Mammalian RuvB DNA Helicase-Like Protein: Homology with Those from Human and Saccharomyces cerevisiael.