Molecular Cloning of Mouse p47, a Second Group Mammalian RuvB DNA Helicase-Like Protein: Homology with Those from Human and Saccharomyces cerevisiael.

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概要

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• A 47k protein (p47) in a high-salt buffer extract of a rat liver nuclear matrix fraction was purified by means of a wheat germ agglutinin affinity column, reversed phase HPLC, and SDS-PAGE, and partial amino acid sequences were analyzed. Based on these sequences, the mouse cDNA of the protein was cloned and sequenced, and its amino acid sequence was deduced. Mouse p47 consists of 463 amino acid residues with a molecular weight of 51, 112. The amino acid sequences of human and Saccharomyces cerevisiae p47s were also deduced from the nucleotide sequences of "expressed sequence tag" fragments and genomic DNA, respectively. These sequences contain helicase motifs and show homology to bacterial RuvB DNA helicases acting in homologous recombination. They also show homology with the putative mammalian helicases p50/TIP49 and RUVBL1. Comparison of the amino acid sequences of p47 group proteins and those of p50/TIP49 group proteins revealed the p47 group proteins to comprise a group distinct from the p50/TIP49 proteins. Ultracentrifugation and gel filtration analyses showed that p47 in the rat liver cytosol fraction exists as large complexes of 697k.

• 社団法人 日本生化学会の論文

著者

• Imai Norio

  Department Of Biochemistry Faculty Of Science Nigata University

• Gohshi Takashi

  Department Of Biochemistry Faculty Of Science Nigata University

• Omata Saburo

  Department Of Biocheaistry Faculty Of Science Niigata University

• Ichimura Tohru

  Department Of Applied Chemistry National Defense Academy

• Shimada Midori

  Department Of Biochemistry Faculty Of Science Nigata University

• Horigome Tsuneyoshi

  Department Of Biocheaistry Faculty Of Science Niigata University

• Kawahire Shigeru

  Course Of Biosystem Science Graduate School Of Science And Techunology

• Imai Norio

  Department of Biochemistry, Faculty of Science, Niigata University

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