A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II : Cell Biology
スポンサーリンク
概要
- 論文の詳細を見る
Here we produced a monoclonal antibody (termed as mH2) which specifically recognized an 86-kD subunit of Human DNA helicase II(p86) in HeLa cells. Immunohistochemical analysis of p86 throughout the cell cycle showed that its localization was changed from the nudei to the cytoplasm, when the cells entered into prophase, and p86 localized diffusely in the cytoplasm during mitosis. Double staining of interphase HeLa cells with mH2 and an antibody, which recognized a component of nuclear pore complex, showed that p86 did not localize at the nuclear envelop in interphase cells.
- 社団法人日本動物学会の論文
- 2000-12-10
著者
-
IWASAKI Takahiro
Department of Biological Science, Graduate School of Science,Hiroshima University
-
MURATA-HORI Maki
Department of Biological Science, Graduate School of Science,Hiroshima University
-
HOSOYA Hiroshi
Department of Biological Science, Graduate School of Science,Hiroshima University
-
岩崎 俊雄
Department Of Biochemistry And Molecular Biology Nippon Medical School
-
Hosoya N
Otsuma Women's University
-
Murata‐hori M
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Murata-hori Maki
Department Of Biological Science Graduate School Of Science Hiroshima University
-
OMATA Saburo
Department of Biochemistry, Faculty of Science, Niigata University
-
HORIGOME Tsuneyoshi
Department of Biochemistry, Faculty of Science, Niigata University
-
Omata Saburo
Department Of Biocheaistry Faculty Of Science Niigata University
-
Hosoya H
Graduate School Of Biological Science Hiroshima University
-
Hosoya Hiroshi
Department Of Biological Science Faculty Of Science Hiroshima University
-
UCHIMURA Takashi
Department of Pharmaceutics, Tohoku College of Pharmacy
-
Uchimura Takashi
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Uchimura Takashi
Department Of Biological Science Graduate School Of Science University Of Hiroshima
-
Hosoya Natsumi
Otsuma Women's University
-
Horigome T
Department Of Biochemistry Faculty Of Science Niigata University
-
Horigome Tsuneyoshi
Department Of Biocheaistry Faculty Of Science Niigata University
-
Iwasaki T
Department Of Life Science Graduate School Of Life Science University Of Hyogo
-
Iwasaki Takahiro
Department Of Biological Science Graduate School Of Science Hiroshima University
-
Iwasaki T
Research Center For Environmental Genomics Kobe University
関連論文
- S72 生理活性物質NOとその生体内における合成制御 : NO合成酵素はいかにして調節されているか?
- Activation of Actin-Activated MgATPase Activity of Myosin II by Phosphorylation with MAPK-Activated Protein Kinase-1b (RSK-2)^1
- 脳神経型一酸化窒素合成酵素活性化/安定化物質の検索
- NO合成酵素ヘム結合ドメインとシトクロムP450とのトポロジーの比較
- 発現した非ヘム鉄欠失キサンチン脱水素酵素の性質
- Toxicity and Bioaccumulation of Hexavalent Chromium in Green Paramecium, Paramecium bursaria
- Phylogeny of Vertebrate Src Tyrosine Kinases Revealed by the Epitope Region of mAb327
- In vitro reconstitution of fertilization signaling by using membrane rafts and cell-free extracts from Xenopus eggs(Symposium on the Mechanism of Signal Transduction in Fertilization: its Diversity, Universality and Evolution)
- Hydrogen peroxide induces Src family tyrosine kinase-dependent activation of Xenopus eggs
- NO合成酵素の構造と機能 (ヘム酵素とガス状メディエーターの分子病態生理--最近のトピックス)
- Inhibition of Neuronal Nitric Oxide Synthase by Phosphatidylinositol 4, 5-Bisphosphate and Phosphatidic Acid
- X-Ray Absorption Spectroscopic Analysis of the High-Spin Ferriheme Site in Substrate-Bound Neuronal Nitric-Oxide Synthase^1
- Sequence Motif-Specific Assignment of Two [2Fe-2S] Clusters in Rat Xanthine Oxidoreductase Studied by Site-Directed Mutagenesis
- Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation
- バキロウイルス発現系によるキサンチン脱水素酵素の蛋白翻訳後の機能発現
- Molecular Shape and ATP Binding Activity of Rat p50, a Putative Mammalian Homologue of RuvB DNA Helicase
- Evidence for Direct Binding of Intracellularly Distributed Ganglioside GM2 to Isolated Vimentin Intermediate Filaments in Normal and Tay-Sachs Disease Human Fibroblasts
- VISUALIZATION OF VISUAL PATHWAYS IN THE ASCIDIAN CIONA INTESTINALIS LARVA USING WHEAT GERM AGGLUTININ (WGA) TRANSGENE(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Malformation of Immature Starfish Oocytes by Theonellapeptolide Ie, a Tridecapeptide Lactone from a Marine Sponge Petrosia Species, through Disturbance of Cortical F-Actin Distribution(Biochemistry & Molecular Biology)
- Glycosylation of the Alpha and Beta Tubulin by Sialyloligosaccharides(Biochemistry)
- Release of a Newly-Identified Cysteine protease, Tetrain, from Tetrahymena into Culture Medium during the Cell Growth^1
- Symbiotic Algae-free Strains of The Green Paramecium Paramecium bursaria Produced by Herbicide Paraquat
- A monoclonal antibody, mH1, specifically recognizes microvilli of dividing HeLa cells
- Purification and Molecular Shape of a 144 kDa Protein Bearig N-Acetylglucosamine residues from Rat Liver Nuclear Envelopes
- Characterization of the Binding of Nuclear Envelope Precursor Vesicles and Chromatin, and Purification of the Vesicles
- Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells
- cDNA Cloning of Nuclear Localization Signal Binding Protein NBP60, a Rat Homologue of Lamin B Receptor, and Identification of Binding Sites of Human Lamin B Receptor for Nuclear Localization Signals and Chromatin^1
- Characterization of p92, Karyopherin β, Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes
- Mitosis-specific Phosphorylation of Smooth Muscle Regulatory Light Chain of Myosin II at Ser-1 and/or -2 and Thr-9 in Sea Urchin Egg Extract
- Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleoprotein K in Xenopus oocytes, eggs, and early embryos
- Association of c-Src with p52Shc in Mitotic NIH3T3 Cells as Revealed by Src-Shc Binding Site-Specific Antibodies
- ANALYSIS OF RECOGNITION SITE FOR SRC-SPECIFIC MONOCLONAL ANTIBODY(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- ACTIVATION MECHANISM OF SRC IN XENOPUS EGG FERTILIZATION(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Lupeol Esters from the Twig Bark of Japanese Pear (Pyrus serotina Rehd.) cv. Shinko
- Effect of Non-Insulin Dependent Diabetes on Cyclosporin A Disposition in Goto-Kakizaki (GK) Rats
- PB-28 A New Bioassy For Acrylamide Toxicity Using Protozoa, Paramecium bursaria(Monitoring,Poster presentation B)
- EFFECTS OF ACRYLAMIDE ON A SYMBIOTIC ASSOCIATION OF SYMBIOTIC ALGAE WITH THE CILIATE PARAMECIUM BURSARIA(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Presence of a Tetrahymena growth promoting activity in fetal bovine serum
- Improved method for coupling of DNase I to a crosslinked agarose gel bead
- Use of Paramecium Species in Bioassays for Environmental Risk Management: Determination of IC_ Values for Water Pollutants
- EFFECT OF 8T STRONG STATIC MAGNETIC FIELD ON BEHAVIOR OF SOME PARAMECIA(Behavior Biology and Ecology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- The Effects of Substituents Introduced into 9-Aminoacridine on Frameshift Mutagenicity and DNA Binding Affinity
- Frameshift Mutagenicity and DNA Intercalation of 9-Amino-2-hydroxyacridine, a Rat Liver S9 Metabolite of 9-Aminoacridine
- Proposed Two-molecule Intercalation between Neighboring Base Pairs of DNA
- Enhancement of Mutagenic Activity of 9-Aminoacridine by Introducing a Nitro Group into the Molecule
- Spectroscopic Evidence in Support of Horseradish Peroxidase Compound II-catalyzed Oxidation of Salicylic Acid but Not of Phenylethylamine(Biochemistry & Molecular Biology)
- Spectroscopic Evidence That Salicylic Acid Converts a Temporally Inactivated Form of Horseradish Peroxidase (Compound III) to the Irreversibly Inactivated Verdohemoprotein (P-670)(Biochemistry & Molecular Biology)
- Diphosphorylated MRLC is Required for Organization of Stress Fibers in Interphase Cells and the Contractile Ring in Dividing Cells
- A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II : Cell Biology
- Molecular Cloning and Splicing Isoforms of Mouse p144, a Homologue of CA150
- Molecular Cloning of Mouse p47, a second Group Mammalian RuvB DNA Helicase-Like Protein : Homology with Those from Human and Saccharomyces cerevisiae^1
- VISUALIZATION OF VISUAL PATHWAY IN ASCIDIAN LARVAE(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- COVALENT PROTEIN MODIFICATIONS IN THE BRAIN OF METHYLNERCURY(MeHg)-TREATED RATS.(Biochemistry)(Proceedings of the Sixty-Second Annual Meeting of the Zoological Society of Japan)
- CHANGES IN PROTEIN PHOSPHORYLATION IN THE BRAIN OF THE RAT AFTER IN VIVO METHYLMERCURY TREATMENT : Biochemistry
- Spatial Localization of Mono- and Diphosphorylated Myosin II Regulatory Light Chain at the Leading Edge of Motile HeLa Cells
- Proline-rich domain in dynamin-2 has a low microtubule-binding activity : how is this activity controlled during mitosis in HeLa cells?
- Activation of Chicken Gizzard Myosin Light Chain Kinase by Ca^/Calmodulin is Inhibited by Autophosphorylation
- Myosin II Regulatory Light Chain as a Novel Substrate for AIM-1, an Aurora/IpIIp-related Kinase from Rat^1
- LOCALIZATION OF PHOSPHORYLATED REGULATORY LIGHT CHAIN OF MYOSIN II DURING CYTOKINESIS(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Cell-cycle-dependent Regulation of Myosin Light Chain Kinase
- EFFECT OF WORTMANNIN, A MYOSIN LIGHT CHAIN KINASE INHIBITOR, ON FIRST CELL DIVISION OF SEA URCHIN EGGS(Cell Biology and Morphology)(Proceedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan)
- FUNCTION OF PHOSPHORYLATED MYOSIN REGULATORY LIGHT CHAIN IN THE CELL(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ROLES OF PHOSPHORYLATED MYOSIN II IN CELL MIGRATION(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Reversed-Phase High-Performance Liquid Chromatography of Membrane Proteins on Perfusion-type Polystyrene Resin Columns in 60% Formic Acid
- Tracheobronchial Amyloidosis in a Patient with Sarcoidosis
- Characterization of p92, Karyopherin .BETA., Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes.
- Molecular Cloning of Mouse p47, a Second Group Mammalian RuvB DNA Helicase-Like Protein: Homology with Those from Human and Saccharomyces cerevisiael.