Toxicity and Bioaccumulation of Hexavalent Chromium in Green Paramecium, Paramecium bursaria

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概要

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• The effects of hexavalent chromium on the cell growth of green paramecium, Paramecium bursaria (P. bursaria), were investigated in this study. Two strains (MB-1 and F_1 generation) of P. bursaria were incubated in lettuce media supplemented with different concentrations of potassium dichromate under light (L) L (24hr light), L dark (LD)(12hr light : 12hr dark) and DD (24hr dark) conditions, and the IC_<50> values were obtained. The IC_<50> 7-day value showed that the toxicity of potassium dichromate was light-sensitive in both strains of P. bursaria. The results of the toxic effect of chromium on the cell shape of P. bursaria (BWK-4) showed that the body ratio of P. bursaria increased, even if the cells were incubated for 24hr with 0.5μM potassium dichromate solution, indicating that the cell shape of P. bursaria is very sensitive to potassium dichromate. The average amount of chromium accumulated in green paramecium ranged from 1.72 to 15.5pg Cr/cell in a time- and concentration-dependent manner. This finding indicates the possibility of the use of P. bursaria as a biomonitor and bioaccumulator for chromium contaminants in aquatic environments. The experiment with electrical stimuli into the culture of P. bursaria indicated that cells accumulated at the negative electrode, suggesting that P. bursaria carried a positive charge. Thus, because the positive charge does not cause any significant absorption of chromium into the cells, P. bursaria may develop a variety of mechanisms for chromium accumulation in the cell. Further research studies are required to elucidate the mechanism of chromium uptake in P. bursaria.

• 2005-12-01

著者

• HOSOYA Hiroshi

  Department of Biological Science, Graduate School of Science,Hiroshima University

• Takahashi Toshiyuki

  Department of Cardiology, JR Tokyo General Hospital

• 高橋 孝行

  北海道大学大学院先端生命科学研究院

• Takahashi T

  Biological Laboratory Nishikyushu University

• 高橋 孝行

  Dep. Of Biophysics And Biochemistry Graduate School Of Sci. Univ. Of Tokyo

• KOSAKA Toshikazu

  Department of Biological Science, Faculty of Science, Hiroshima University

• Ueki Tatsuya

  Department of Zoology, Graduate School of Science, Kyoto University

• Michibata Hitoshi

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Hosoya H

  Graduate School Of Biological Science Hiroshima University

• Hosoya Hiroshi

  Department Of Biological Science Faculty Of Science Hiroshima University

• Ueki Tatsuya

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Takahashi Toshiyuki

  Department Of Biology Keio University

• Kosaka Toshikazu

  Graduate School of Biological Science, Hiroshima University

• Mortuza M.

  Department of Biological Science, Graduate School of Science, Hiroshima University

• Kosaka T

  Graduate School Of Biological Science Hiroshima University

• Mortuza M.

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Kosaka Toshikazu

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Takahashi Toshiyuki

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Takahashi Takayuki

  Department Of Biophysics And Biochemistry Graduate School Of Science University Of Tokyo

• Takahashi Toshiyuki

  Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University

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