Spectroscopic Evidence in Support of Horseradish Peroxidase Compound II-catalyzed Oxidation of Salicylic Acid but Not of Phenylethylamine(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Salicylic acid and phenylethylamine are putative substrates for naturally occurring reactions for generation of reactive oxygen species, which are catalyzed by plant peroxidases. Here, we used commercially available highly purified horseradish peroxidase-C (HRP-C) as a model enzyme for spectroscopic analysis, and obtained data suggesting that the Compound II form of HRP-C does not utilize phenylethylamine as substrate. In contrast, addition of salicylic acid to Compound II resulted in rapid conversion of Compound II to the native form.
- 社団法人日本農芸化学会の論文
- 2002-03-23
著者
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HOSOYA Hiroshi
Department of Biological Science, Graduate School of Science,Hiroshima University
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ADACHI Motoyasu
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
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Takuwa K
Suntory Institute For Bioorganic Research
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Adachi Motoyasu
Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University
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Muto S
Bioscience Center Nagoya University
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Kawano T
Dep. Of Biological And Environmental Chemistry Fac. Of Agriculture Tottori Univ.
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Hosoya H
Graduate School Of Biological Science Hiroshima University
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Hosoya Hiroshi
Department Of Biological Science Faculty Of Science Hiroshima University
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Adachi M
Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University
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KAWANO Tomonori
UMR INRA-UHP Interactions Arbre
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MUTO Shoshi
Micro-Organismes, Centre Institut National de la Recherche Agronomique de Nancy
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ADACHI Masaru
Nagoya University Bioscience Center
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LAPEYRIE Frederic
Department of Biological Science, Graduate School of Science and PRESTO-JST, Hiroshima University
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Kawano T
Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University
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Kawano Tsuyoshi
Dep. Of Bioresource Sciences Fac. Of Agriculture Tottori Univ.
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Kawano Tsuyoshi
Department Of Biological And Environmental Chemistry Tottori University
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Lapeyrie F
Department Of Biological Science Graduate School Of Science And Presto-jst Hiroshima University
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Kono Takaharu
Department Of Agricidtural Chemistry Faculty Of Agriculture The University Of Tokyo
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