An Altered Method of Feeding RNAi That Knocks Down Multiple Genes Simultaneously in the Nematode Caenorhabditis elegans
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概要
- 論文の詳細を見る
In reverse genetics, RNA interference (RNAi) which is substitutable for gene-disruption, is an outstanding method for knockdown of a gene’s function. In Caenorhabditis elegans, feeding RNAi is most convenient, but this RNAi is not suitable for knockdown of multiple genes. Hence, we attempted to establish an efficient method of feeding RNAi for multiple knockdown. We produced bacteria yielding three distinct double-stranded RNAs bound to one another, and fed those bacteria to C. elegans. Quantitative RT-PCR and observation of phenotypes indicated that our method is much more efficient than the traditional one. Our method is useful for investigating genes’ functions in C. elegans.
- 社団法人 日本農芸化学会の論文
- 2010-11-23
著者
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Kawano Tsuyoshi
Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University
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Kawano Tsuyoshi
Dep. Of Bioresource Sciences Fac. Of Agriculture Tottori Univ.
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Kawano Tsuyoshi
Department Of Biological And Environmental Chemistry Tottori University
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Iwasaki Takashi
Department Of Bioresource Sciences Faculty Of Agriculture Tottori University
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Gouda Kenji
Department Of Bioresource Sciences Faculty Of Agriculture Tottori University
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Matsunaga Yohei
Department Of Bioresources Science The United Graduated School Of Agricultural Sciences Tottori Univ
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Matsunaga Yohei
Department Of Bioresource Sciences Faculty Of Agriculture Tottori University
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