Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker's Yeast and Deoxyriboaldolase-Expressing Escherichia coli
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概要
- 論文の詳細を見る
2-Deoxyribose 5-phosphate production through coupling of the alcoholic fermentation system of baker’s yeast and deoxyriboaldolase-expressing Escherichia coli was investigated. In this process, baker’s yeast generates fructose 1,6-diphosphate from glucose and inorganic phosphate, and then the E. coli convert the fructose 1,6-diphosphate into 2-deoxyribose 5-phosphate via D-glyceraldehyde 3-phosphate. Under the optimized conditions with toluene-treated yeast cells, 356 mM (121 g/l) fructose 1,6-diphosphate was produced from 1,111 mM glucose and 750 mM potassium phosphate buffer (pH 6.4) with a catalytic amount of AMP, and the reaction supernatant containing the fructose 1,6-diphosphate was used directly as substrate for 2-deoxyribose 5-phosphate production with the E. coli cells. With 178 mM enzymatically prepared fructose 1,6-diphosphate and 400 mM acetaldehyde as substrates, 246 mM (52.6 g/l) 2-deoxyribose 5-phosphate was produced. The molar yield of 2-deoxyribose 5-phosphate as to glucose through the total two step reaction was 22.1%. The 2-deoxyribose 5-phosphate produced was converted to 2-deoxyribose with a molar yield of 85% through endogenous or exogenous phosphatase activity.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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小川 順
京大院・農・応用生命
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Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Sakai Takafumi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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斉藤 謙一
北海道薬科大学
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Sakai T
Kyoto Univ. Kyoto Jpn
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Shimizu S
Agricultural Sciences Kyoto University
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Saito K
Department Of Kampo Medicinal Science Hokkaido College Of Pharmacy
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Takuwa K
Suntory Institute For Bioorganic Research
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Mikami Y
Chiba Univ. Chiba Jpn
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Sakai T
Kikkoman Corp. Chiba Jpn
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HORINOUCHI Nobuyuki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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KAWANO Takako
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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SAITO Kyota
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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MATSUMOTO Seiichiro
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
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SASAKI Mie
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
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MIKAMI Yoichi
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
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斉藤 謙一
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
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Sakai Toshio
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Takagi S
Ehime Prefectural Fisheries Experimental Station
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Sasaki M
Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.
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Kawano T
Dep. Of Biological And Environmental Chemistry Fac. Of Agriculture Tottori Univ.
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Mikami Yoichi
Tobacco Science Research Laboratory
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Mikami Yoichi
Central Research Institute The Japan Tobacco 〓 Salt Public Corporation
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Shimizu S
Iwate Univ. Iwate Jpn
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Saguchi Ken-ichi
Laboratory Of Biopharmaceutics School Of Pharmaceutical Sciences Showa University
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Shinozaki Kazuo
Riken Kanagawa Jpn
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Kawano T
Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University
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Mikami Y
Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.
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Sasaki Kanako
Riken Kanagawa Jpn
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Kawano Tsuyoshi
Dep. Of Bioresource Sciences Fac. Of Agriculture Tottori Univ.
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Kawano Tsuyoshi
Department Of Biological And Environmental Chemistry Tottori University
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Kono Takaharu
Department Of Agricidtural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Nobuyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Saito Ken-ichi
Department Of Toxicology And Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Sakoda K
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hoshi University
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