Bilirubin Dehydrogenase, an Enzyme in Aspergillus ochraceus IB-3 Useful for Diagnostic Measurement of Bilirubin(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Bilirubin dehydrogenase, a membrane-bound enzyme that catalyzes the one-step oxidation of ditaurobilirubin and bilirubin to ditaurobiliverdin and biliverdin, respectively, in the presence of an electron acceptor, was found in Aspergillus ochraceus IB-3, and purified from the membrane fraction through solubilization by Triton X-100. Phenazine and quinone derivatives acted as electron acceptors. Accumulation of ditaurobiliverdin and biliverdin by enzyme catalysis increased the absorbance at 660 nm, which is far from the range of wavelengths affected by serum ingredients. The enzyme selectively oxidized ditaurobilirubin at low pH, so changes in the reaction pH enable the enzyme to discriminate between the bilirubin fractions ditaurobilirubin (an example of conjugated bilirubin) and bilirubin (an example of unconjugated bilirubin). Using the enzyme, 2 to 80 μM of ditaurobilirubin were measured accurately by monitoring the changes in absorbance at 660 nm.
- 社団法人日本農芸化学会の論文
- 2002-11-23
著者
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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KANO Kenji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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IKEDA Tokuji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Tanaka H
Faculty Of Pharmacy Meijo University
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Kano Kenji
Kyoto Univ. Kyoto Jpn
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Kano Kenji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Ikeda Tokuji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Tanaka H
Department Of Agricultural Chemistry Faculty Of Agriculture Utsunomiya University
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kano Kenji
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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Tanaka H
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tanaka H
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Toyama Hirohide
Institute For Chemical Research Kyoto University
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Tanaka Haruyuki
Chemical Branch Faculty Of Engineering And Kansai University High Technology Research Center Kansai
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Kano Kenji
Division Of Applied Life Sciences Laboratory Of Bio-analytical And Physical Chemistry Graduate Schoo
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Sulistyaningyah Woro
Division Of Applied Life Sciences Laboratory Of Fermentation Physiology And Applied Microbiology Gra
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TANAKA Hiromi
Division of Applied Life Sciences, Laboratory of Fermentation Physiology and Applied Microbiology, G
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Tanaka Hiroyoki
Faculty Of Pharmaceutical Sciences Kyushu University
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Shimizu Sakayu
Division Of Applied Life Sciences Laboratory Of Fermentation Physiology And Applied Microbiology Gra
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Kano Kenji
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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TANAKA Hiromi
Department of Physics Faculty of Education, Saga University
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Sulistyaningdyah Woro
Division of Applied Life Sciences, Laboratory of Fermentation Physiology and Applied Microbiology, Graduate School of Agriculture, Kyoto University
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