Purification and Characterization of a New Aldehyde Oxidase from Pseudomonas sp. AIU 362(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
An aldehyde oxidase exhibiting high activity on glyoxal was purified to an electrophoretically homogenous state from Pseudomonas sp. AIU 362, which was isolated from a soil sample using a methoxyethanol medium. The enzyme oxidized not only glyoxal but also short-chain aliphatic aldehydes and aromatic aldehydes. Thus, this enzyme was classified into the aldehyde oxidase (ALOD) group. However, it was composed of four identical subunits with a molecular mass of 27kDa, whereas other microbial ALODs were composed of three hetero subunits, and ALODs from plant and animals were composed of two identical subunits. The NH_2-terminal sequence also showed no similarity to that of other ALODs. These results indicate that ALOD from Pseudomonas sp. AIU 362 is a new aldehyde oxidase. This ALOD was induced by 2-methoxyethanol, methanol or isopropanol.
- 2008-09-25
著者
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Sasaki Yasutaka
The United Graduate School of Agricultural Sciences, Iwate University
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Iwasaki Akira
Life Science R & D Center, Kaneka Co.
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Hasegawa Junzo
Life Science R & D Center, Kaneka Co.
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Isobe Kimiyasu
The United Graduate School of Agricultural Sciences, Iwate University
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清水 昌
京都大学大学院農学研究科
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清水 昌
近畿大学 農
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Isobe Kimiyasu
Department of Agro-bioscience, Faculty of Agriculture, Iwate University
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Kataoka M
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kataoka Michihiko
Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.
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Kataoka Michihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Isobe Kimiyasu
The United Graduate School Of Agricultural Sciences Iwate University
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Isobe Kimiyasu
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Hasegawa J
Life Science R & D Center Kaneka Co.
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Sasaki Yasutaka
The United Graduate School Of Agricultural Sciences Iwate University
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Shimazu S
Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.
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Shimizu S
Agricultural Sciences Kyoto University
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Shimizu S
Kyoto Univ. Kyoto Jpn
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Shimizu Sakayu
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Hasegawa J
Ichimaru Pharcos Co. Ltd. Gifu Jpn
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Hasegawa Junzo
Life Science Rd Center Kaneka Corporation
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Iwasaki Akira
Life Science R & D Center Kaneka Co.
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shiozaki Shozo
Department Of Agricultural Chemistry Kyoto University:(present Office)life Science Institute Nippon
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Kataoka Michihiko
Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture
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Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
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Hasegawa Junzo
Life Science R & D Center Kaneka Co.
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清水 晶
京都大学 大学院
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Shimizu Sakayu
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Shimizu S
Iwate Univ. Iwate Jpn
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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