Production and characterization of alcohol oxidase from Penicillium purpurescens AIU 063(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• A filamentous fungus belonging to Penicillium purpurescens was isolated as a strain grown well in methanol medium. The strain produced an alcohol oxidase (AOD), which oxidizes short-chain primary alcohols and ethylene glycol, by incubation with a medium containing not only methanol, but also ethanol, ethylene glycol, glycerol or glucose. This AOD belonged to the same group as AOD from methylotrophic yeast, but some properties were different from AODs from methylotrophic yeast and other fungi. Thus, this enzyme consisted of four identical subunits of 66kDa, while AODs from methylotrophic yeasts were of eight subunits. The enzyme activity was enhanced to two-fold by incubation at pH 6.0 and 40℃ for 60min, whereas the K_m values for ethanol and ethylene glycol did not change.

著者

• Shimizu Sakayu

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Kataoka Michihiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Ogawa Jun

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Isobe Kimiyasu

  The United Graduate School of Agricultural Sciences, Iwate University

• 清水 昌

  京都大学大学院農学研究科

• 清水 昌

  近畿大学 農

• Isobe Kimiyasu

  Department of Agro-bioscience, Faculty of Agriculture, Iwate University

• Takahashi Toyofumi

  Department of Agro-bioscience, Iwate University

• Kataoka M

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Kataoka Michihiko

  Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.

• Kataoka Michihiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Isobe Kimiyasu

  The United Graduate School Of Agricultural Sciences Iwate University

• Isobe Kimiyasu

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• Shimazu S

  Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.

• Shimizu S

  Agricultural Sciences Kyoto University

• Shimizu S

  Kyoto Univ. Kyoto Jpn

• Shimizu Sakayu

  Division Of Applied Life Science Graduate School Of Agriculture Kyoto University

• Ogawa Jun

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Shiozaki Shozo

  Department Of Agricultural Chemistry Kyoto University:(present Office)life Science Institute Nippon

• Kataoka Michihiko

  Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture

• 清水 晶

  京都大学 大学院

• Shimizu Sakayu

  Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra

• Shimizu S

  Iwate Univ. Iwate Jpn

• 清水 昌

  京都大学応用生命科学専攻

• Takahashi Toyofumi

  Department Of Agro-bioscience Iwate University

• Kataoka Michihiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

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