Identification of Mutation Sites on Δ6 Desaturase Genes from Mortierella alpina 1S-4 Mutants
スポンサーリンク
概要
- 論文の詳細を見る
Three Δ6 desaturase-defective mutants, designated YB214, HR95, and ST66, were newly isolated from Mortierella alpina 1S-4, after treating wild-type spores with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). These three mutants and Mut49, isolated previously, are capable of accumulating 5,11,14-cis-eicosatrienoic acid (20:3Δ5). Two functional Δ6 desaturases (Δ6I and Δ6II) were found to exist in M. alpina 1S-4. The mutation sites on the Δ6I gene in the Δ6 desaturase-defective mutants were identified. The mutations each resulted in an amino acid replacement (W314Stop, T375K, and G390D) in Δ6I from ST66, HR95, and YB214 respectively, and uncorrected transcription of the Δ6I gene in Mut49 was caused by disappearance of the GT-terminal of the second intron, resulting in low Δ6 desaturation activity in these mutants. On the other hand, there was no mutation site on the Δ6II genes of the mutants.
- 社団法人 日本農芸化学会の論文
- 2005-05-23
著者
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Sakuradani Eiji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Shimizu Sakayu
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Abe Takahiro
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Sakuradani Eiji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sakuradani E
Kyoto Univ. Kyoto Jpn
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Shimizu S
Agricultural Sciences Kyoto University
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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ASANO Takahiro
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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KANAMARU Hiroyuki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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IOKA Yuji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ioka Yuji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Abe Takahiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sakuradani Eiji
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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Kanamaru Hiroyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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